Summary
The induction of yeast cell aggregates in a column reactor was initiated by packing yeast cell paste of Saccharomyces uvarum into the column, and then YMP broth was fed into the column from the bottom at a linear flow rate of 2.5 cm/h. Thereafter, yeast cells aggregated in the column within 48 h without a supply of oxygen. When this yeast aggregate column reactor was used for continuous ethanol production, a final ethanol concentration of 10.8% (w/v) was obtained from 23% (w/v) of glucose in a YMP broth with a dilution rate of 0.05 h-1, and 4.9% (w/v) was obtained from 10% (w/v) of glucose with a dilution rate of 0.6 h-1. The theoretical yield was above 97% in both cases. The ethanol production rates were 13 g1 h-1 l-1 and 90 g1 h-1 l-1 for producing 10.8% (w/v) and 4.9% (w/v) of ethanol respectively. This column reactor was maintained at a steady state for more than one month.
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Chen, L., Gong, CS. Continuous ethanol production using induced yeast aggregates. Appl Microbiol Biotechnol 25, 208–212 (1986). https://doi.org/10.1007/BF00253650
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DOI: https://doi.org/10.1007/BF00253650