Skip to main content
Log in

Saccharification of cellulosics by Microbispora bispora

  • Applied Microbiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

The saccharification efficiency of cellulase from the thermophilic actinomycete Microbispora bispora was evaluated using commercially available feedstocks. The enzyme preparation was effective against refuse derived cellulose with 30% being converted to glucose in a 24 hour period. Pretreatment of the refuse with cadoxen resulted in an increase in saccharification efficiency to 70%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bungay HR (1981) Energy — the biomass option. John Wiley and Sons, New York

    Google Scholar 

  • Chabel DS, MooYoung M, Vlach D (1981) Effect of physical and physiochemical pretreatments of wood for SCP production with Chaetomium cellulyticum. Biotech Bioeng 23:2417–2420

    Google Scholar 

  • Dale BE, Moreira MJ (1982) A freeze explosion technique for increasing cellulosic hydrolysis. Biotech Bioeng Symp 4:31–43

    Google Scholar 

  • Eveleigh DE (1982) The fermentation of biomass — current aspects. In: Cote W (ed) Biomass utilization, NATO Advanced Study Institute, Lisbon, Portugal. Plenum Press, New York

    Google Scholar 

  • Hägerdal BGR, Ferchak JD, Pye EK (1978) Cellulolytic enzyme system of Thermoactinomyces sp. grown on microcrystalline cellulose. Appl Environ Microbiol 36:606–612

    Google Scholar 

  • Holtzapple MT (1981) The pretreatment and saccharification of poplar wood. Ph. D. Thesis, Lehigh U., Bethlehem, PA

  • Hughes RE, Yablonsky MD, Eveleigh DE (1985) Microbispora bispora: a new source of cellulase. Bull NJ Acad Sci 30:49 (abstract)

    Google Scholar 

  • Jayme G, Lang F (1963) Cellulose solvents. In: Whistler RL (ed) Methods in carbohydrate chemistry, vol. 3, Academic Press, New York London, p 80

    Google Scholar 

  • Katzen R (1980) The alcoholic pulping and recovery process. Chem Eng Prog (Feb. 80): 62–67

    Google Scholar 

  • Ladisch MR, Ladisch CM, Tsao GT (1978) Cellulose to sugars: New path gives quantitative yield. Science 201:743–745

    Google Scholar 

  • Mandels M, Andreotti R, Roche C (1976) Measurement of saccharifying cellulase. In: Gaden EL et al. (eds) Enzymatic conversion of cellulosic materials: technology and applications. Biotech Bioeng Symp 6:21–33

  • Ng T, Busche RM, McDonald CC, Hardy RWF (1983) Production of feedstock chemicals. Science 219:733–740

    Google Scholar 

  • Reese ET, Mandels M (1984) Rolling with the times: production and applications of Trichoderma reesei cellulase. In: Tsao G (ed), Ann Rep Ferm Proc, vol 7, Academic Press, New York, pp 1–20

    Google Scholar 

  • Sarkanen KV (1980) Acid catalyzed delignification of lignocellulose in organic solvents. In: Sarkanen KV, Tillman DA (eds), Progress in biomass conversion, Academic Press, New York, pp 127–144

    Google Scholar 

  • Tsao GT, Chiang LC (1983) Cellulose and hemicellulose technology. In: Smith JE, Berry DR, Kristiansen B (eds) The filamentous fungi, vol 4, Edward Arnold, London, pp 298–326

    Google Scholar 

  • Updegraff DM (1969) Semimicro determination of cellulose in biological materials. Analyt Biochem 32:420–424

    Google Scholar 

  • Waldron CR, Becker-Vallone CA, Eveleigh DE (1986) Isolation and characterization of a cellulolytic actinomycete, Microbispora bispora. Appl Microbiol Biotechnol 24:477–486

    Google Scholar 

  • Walseth CS (1952) Influence of fine structure of cellulose on the action of cellulases. Tappi 35:225–243

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Waldron, C.R., Eveleigh, D.E. Saccharification of cellulosics by Microbispora bispora . Appl Microbiol Biotechnol 24, 487–492 (1986). https://doi.org/10.1007/BF00250328

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00250328

Keywords

Navigation