Abstract
Restriction fragment length polymorphisms of cytoplasmic DNAs and nuclear rDNA were analyzed in several Cichorium intybus genotypes, comprising four white inbred lines, eight red witloof experimental lines, and a number of F1 hybrids derived from two white parents. Chloroplast and mitochondrial restriction patterns led to the distinction between two different cytoplasms, called I and II. Southern hybridization using a nuclear rDNA probe revealed that all the lines possessed two types of rDNA repeat units. The shortest unit was 10 kb and was common to all lines. The largest rDNA repeat unit was 10.5 kb in lines I and 10.4 kb in lines II. In addition, a sequence heterogeneity between the 10.5 and 10.4-kb rDNA repeat units was revealed by Sac I digestion. A 10-kb rDNA unit was successively cloned, mapped, and used as a probe to check the genetic purity of F1 hybrid seeds between line I and II white parents. We found a 30% average percentage of impurities, originating both from selfing and full-sib crossing, in different open-pollinated hybrid samples.
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References
Beckman JS (1988) Oligonucleotide polymorphisms: a new tool for genomic genetics. Biotechnology 6:1061–1065
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Bookjans G, Stummann BM, Henningsen KW (1984) Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength. Anal Biochem 141:244–247
Chétrit P, Mathieu C, Vedel F, Pelletier G, Primard C (1985) Mitochondrial DNA polymorphism induced by protoplast fusion in Cruciferae. Theor Appl Genet 69:361–366
Chétrit P, Rios R, De Paepe R, Vitart V, Gutierres S, Vedel F (1992) Cytoplasmic male sterility is associated with large deletions in the mitochondrial DNA of two Nicotiana sylvestris protoclones. Curr Genet 21:131–137
Courcel AGL de (1989) L'exploitation du polymorphisme moléculaire de 1'ADN: un nouvel outil pour la selection des espèces potagères. Thèse Doct. Univ. Paris Sud, Orsay
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation version II. Plant Mol Biol Rep 1:19–21
Flavell RB (1986) The structure and control of expression of ribosomal RNA genes. Oxford Surv Plant Mol Cell Biol 3:251–274
Grunstein M, Hogness DS (1975) Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72:3961–3965
Levesque H, Vedel F, Mathieu C, de Courcel AGL (1990) Identification of a short rDNA spacer sequence highly specific for a tomato line containing a Tm-1 gene introgressed from Lycopersicon hirsutum. Theor Appl Genet 80:602–608
Millecamps JL (1989) Sélection de chicorées Cichorium intybus L. var. Witloof résistantes aux sulfonylurées par cultures cellulaires. Thése Doct., Univ. Lille Flandres Artois
Rambaud C, Dubois J, Vasseur J (1993) Male-sterile chicory cybrids obtained by intergeneric protoplast fusion. Theor Appl Genet 87:347–352
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
San JH, Vedel F, Sihachakr D, Rémy R (1990) Morphological and molecular characterization of fertile tetraploid somatic hybrids produced by protoplast electrofusion and PEG-induced fusion between Lycopersicon esculentum Mill and Lycopersicon peruvianum Mill. Mol Gen Genet 221:17–26
Till-Bottraud I, Reboud X, Brabant P, Lefranc M, Rherissi B, Vedel F, Darmency H (1992) Outcrossing and hybridization in wild and cultivated foxtail millets: consequences for the release of transgenic crops. Theor Appl Genet 83:940–946
Vedel F, Quétier F, Bayen M (1976) Specific cleavage of chloroplast DNA from higher plants by Eco RI restriction nuclease. Nature 263:440–442
Vermeulen A, Vaucheret H, Pautot V, Chupeau Y (1992) Agrobac-terium-mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (Cichorium intybus L) Plant Cell Rep 11:243–247
Vermeulen A, Desprez B, Lancelin D, Bannerot H (1994) Relationships among Cichorium species and related genera as determined by analysis of mitochondrial RFLPs. Theor Appl Genet 88:159–166
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Communicated by D. R. Pring
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Bellamy, A., Mathieu, C., Vedel, F. et al. Cytoplasmic DNAs and nuclear rDNA restriction fragment length polymorphisms in commercial witloof chicories. Theoret. Appl. Genetics 91, 505–509 (1995). https://doi.org/10.1007/BF00222980
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DOI: https://doi.org/10.1007/BF00222980