Abstract
Construction of a detailed RFLP linkage map of B. rapa (syn. campestris) made it possible, for the first time, to study individual genes controlling quantitative traits in this species. Ninety-five F2 individuals from a cross of Chinese cabbage cv ‘Michihili’ by Spring broccoli were analyzed for segregation at 220 RFLP loci and for variation in leaf, stem, and flowering characteristics. The number, location, and magnitude of genes underlying 28 traits were determined by using an interval mapping method. Zero to five putative quantitative trait loci (QTL) were detected for each of the traits examined. There were unequal gene effects on the expression of many traits, and the inheritance patterns of traits ranged from those controlled by a single major gene plus minor genes to those controlled by polygenes with small and similar effects. The effect of marker locus density on detection of QTL was analyzed, and the results showed that the number of QTL detected did not change when the number of marker loci used for QTL mapping was decreased from 220 to 126; however, a further reduction from 126 to 56 caused more than 15% loss of the total QTL detected. The detection of putative minor QTL by removing the masking effects of major QTL was explored.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beckmann JS, Soller M (1986) Restriction fragment length polymorphisms and genetic improvement of agricultural species. Euphytica 35:111–124
Chyi Y-S, Hownecke ME, Sernyk JL (1992) A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn. campestris). Genome 35:746–757
Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigation of quantitative trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125
Edwards MD, Helentjaris T, Wright S, Stuber CW (1992) Molecular-marker-facilitated investigations of quantitative trait in maize. 4. Analysis based on genome saturation with isozyme and restriction fragment length polymorphism markers. Theor Appl Genet 83:765–774
Ferreira ME, Williams PH, Osborn TC (1994) RFLP mapping of Brassica napus using F1-derived doubled haploid lines. Theor Appl Genet (in press)
Figdore SS, Kennard WC, Song KM, Slocum MK, Osborn TC (1988) Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75:833–840
Keim P, Diers BW, Olson TC, Shoemaker RC (1990) RFLP mapping in soybean: association between marker loci and variation in quantitative traits. Genetics 126:735–742
Keim P, Diers W, Shoemaker RC (1991) Genetic analysis of soybean hard seededness with molecular markers. Theor Appl Genet 79:465–469
Kennard WC, Slocum MK, Figdore SS, Osborn TC (1994) Genetic analysis of morphological variation in Brassica oleracea using molecular markers. Theor Appl Genet 87:721–732
Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Landry B, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991) A genetic map of Brassica napus based on restriction fragment length polymorphism detected with expressed DNA sequences. Genome 34:543–552
Landry B, Hubert N, Crete R, Chang MS, Lincoln S, Etoh T (1992) A genetic map of Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora Brassicae (Woronin). Genome 35:409–420
Lincoln SE, Lander ES (1990) Mapping genes controlling quantitative traits using MAPMAKER/QTL. Whitehead Institute for Biomedical Research, Technical Report, Cambridge, MA
Nienhuis JT, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803
Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphisms linked to genes controlling soluble solid content in tomato fruit. Theor Appl Genet 73:350–356
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726
Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: Comparison across species, generations, and environments. Genetics 127:181–197
SAS Institute Inc (1982) SAS user's guide: basics and statistics. SAS Institute, Cary, N.C.
Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64
Song KM, Osborn TC, Williams PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). 2. Preliminary analysis of subspecies within B. rapa (syn campestris) and B. oleracea. Theor Appl Genet 76:593–600
Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of B. rapa (syn. campestris) based on restriction fragment length polymorphism loci. Theor Appl Genet 82:296–304
Stuber CW, Edwards MD, Wendel JF (1987) Molecular-marker-facilitated investigation of quantitative trait loci in maize. II. Factors influencing yield and its component traits. Crop Sci 27:639–648
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264
Williams PH (1985) Crucifer Genetics Cooperative: resource book. Crucifer Genetics Cooperative, Madison, Wis.
Author information
Authors and Affiliations
Additional information
Communicated by J. S. Beckmann
Rights and permissions
About this article
Cite this article
Song, K., Slocum, M.K. & Osborn, T.C. Molecular marker analysis of genes controlling morphological variation in Brassica rapa (syn. campestris). Theoret. Appl. Genetics 90, 1–10 (1995). https://doi.org/10.1007/BF00220989
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220989