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A tripartite culture system for endomycorrhizal inoculation of micropropagated strawberry plantlets in vitro

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Abstract

The objective of the current investigation was to develop a reliable method to obtain vesicular arbuscular mycorrhizae (VAM) in micropropagated plantlets and to determine their influence on growth. An in vitro system for culturing the VA mycorrhizal fungus Glomus intraradices with Ri T-DNA-transformed carrot roots or nontransformed tomato roots was used in this study as a potential active source of inoculum for the colonization of micropropagated plantlets. After root induction, micropropagated plantlets grown on cellulose plugs (sorbarod) were placed in contact with the primary mycorrhizae in growth chambers enriched with 5000 ppm CO2 and fed with a minimal medium. After 20 days of tripartite culture, all plantlets placed in contact with the primary symbiosis were colonized by the VAM fungus. As inoculum source, 30-day-old VA mycorrhizal transformed carrot roots had a substantially higher infection potential than 5-, 10-or 20-day-old VAM. Colonized plantlets had more extensive root systems and better shoot growth than control plants. The VAM symbiosis reduced the plantlet osmotic potential. This response may be a useful pre-adaptation for plantlets during transfer to the acclimatization stage.

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References

  • Allen MF, Boosalis MG (1983) Effect of two species of VAM fungi on drought tolerance of winter wheat. New Phytol 93:67–76

    Google Scholar 

  • Allen MF, Moore TS Jr, Christinsen M (1982) Phytohormone changes in Bouteloua gracilis infected by vesicular-arbuscular mycorrhizae. II, Altered levels of gibberellin-like substances and abscisic acid in the host plant. Can J Bot 60:468–471

    Google Scholar 

  • Ames RE, Reid CPP, Porter LK, Cambardella C (1983) Hyphal uptake and transport of nitrogen from two 15N-labelled source by Glomus mossae, a vesicular arbuscular mycorrhizal fungus. New Phytol 95:381–396

    Google Scholar 

  • Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (1989) Handbook of plant cell culture, vol 5. Ornamental species. McGraw-Hill, New York

    Google Scholar 

  • Augé RM (1989) Do VA mycorrhizae enhance transpiration by affecting host phosphorus content. J Plant Nutr 12:743–753

    Google Scholar 

  • Augé RM, Schekel KA, Wample RL (1986) Osmotic adjustment in leaves of VA mycorrhizal and non-mycorrhizal rose plants in response to drought stress. Plant Physiol 82:765–770

    Google Scholar 

  • Augé RM, Schekel KA, Wample RL (1987) Rose leaf elasticity changes in response to mycorrhizal colonization and drought acclimation. Plant Physiol 70:175–182

    Google Scholar 

  • Azcon R, Ocampo JA (1984) Effect of root exudation on VA mycorrhizal infection at early stages of plant growth. Plant Soil 82:133–138

    Google Scholar 

  • Bécard G, Fortin JA (1988) Early events of vesicular-arbuscular mycorrhiza formation on Ri T-DNA transformed roots. New Phytol 108:211–218

    Google Scholar 

  • Bécard G, Piché Y (1989) Fungal growth stimulation by CO2 and root exudates in vesicular-arbuscular mycorrhizal symbiosis. Appl Environ Microbiol 55:2320–2325

    Google Scholar 

  • Bécard G, Piché Y (1992) Establishment of VA mycorrhizae in root organ culture: review and proposed methodology. In: Varna A, Norris JR, Read DJ (eds) Methods in microbiology: experiments with mycorrhizae. Academic Press, New York, pp 89–108

    Google Scholar 

  • Biermann B, Linderman RG (1983) Increased geranium growth using pretransplant inoculation with a mycorrhizal fungus. J Am Soc Hortic Sci 108:972–976

    Google Scholar 

  • Brundrett MC, Piché Y, Peterson RL (1984) A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can J Bot 62:2128–2134

    Google Scholar 

  • Chabot S, Bécard G, Piché Y (1992a) Life cycle of Glomus intraradices in root organ culture. Mycologia 84:315–321

    Google Scholar 

  • Chabot S, Bel-rhild R, Chénevert R, Piché Y (1992b) Hyphal growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker & Hall, by the activity of structurally specific flavonoid compounds under CO2-enriched conditions. New Phytol 122:461–467

    Google Scholar 

  • Chapman DS, Augé RM (1994) Physiological mechanisms of drought resistance in four native ornamental perennials. J Am Soc Hortic Sci 119:299–306

    Google Scholar 

  • Chavez MC, Ferrara-Cerato R (1990) Effect of vesicular-arbuscular mycorrhizae on tissue cultured plantlets of strawberry. HortScience 25:903–905

    Google Scholar 

  • Dehne HW (1982) Interaction between VAM fungi and plant pathogens. Phytopathology 72:1115–1119

    Google Scholar 

  • Desjardins Y, Gosselin A, Yelle S (1987) Acclimatization of ex vitro strawberry plantlets in CO2 enriched environments and supplementary lighting. J Am Hortic Sci 112:846–851

    Google Scholar 

  • Desjardins Y, Laforge F, Lussier C, Gosselin A (1988) Effect of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hortic 230:45–53

    Google Scholar 

  • Desjardins Y, Gosselin A, Lamarre M (1990) Growth of transplants and in vitro cultured clone of asparagus in response to CO2 enrichment and supplemental lighting. J Am Soc Hortic Sci 115: 364–368

    Google Scholar 

  • Donnelly DJ, Vidaver WE, Lee KY (1985) The anatomy of tissue cultured red raspberry prior to and after transfer to soil. Plant Cell Tissue Organ Cult 4:43–50

    Google Scholar 

  • Ferguson JJ, Menge JA (1982) The influence of light intensity and artificially extended photoperiod upon infection and sporulation of Glomus fasciculatus on sudangrass and on root exudation of sudangrass. New Phytol 92:183–191

    Google Scholar 

  • Friese CF, Allen MF (1991) The spread of VA mycorrhizal fungal hyphae in the, soil: inoculum types and external hyphal architecture. Mycologia 83:409–418

    Google Scholar 

  • Hasegawa PH, Murashige T, Takatori FH (1973) Propagation of asparagus through shoot apex culture. II. Light temperature requirements, transplantability of plant, and cytohistological characteristics. J Am Soc Hortic Sci 98:143–148

    Google Scholar 

  • Hayman DS (1983) The physiology of VA endomycorrhizal symbiosis. Can J Bot 61:944–963

    Google Scholar 

  • Hdider C, Desjardins Y (1994) Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets. Plant Cell Tissue Organ Cult 36:27–33

    Google Scholar 

  • Hepper CM (1981) Techniques for studying the infection of plants by vesicular-arbuscular mycorrhizal fungi under axenic conditions. New Phytol 88:641–647

    Google Scholar 

  • Kartha KK, Leung NL, Pahl K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J Am Soc Hortic Sci 105:481–484

    Google Scholar 

  • Koide R (1985) The effect of VAM infection and phosphorus staus on sunflower hydraulic and stomatal properties. J Exp Bot 36:1087–1098

    Google Scholar 

  • Kozai T, Iwanami Y (1988) Effect of CO2 enrichments and sucrose concentration under high photon flux on tissue cultured plantlets growth of carnation (Dianthus caryophyllus L.) during the preparation stage. J Japan Soc Hortic Sci 57:255–264

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K, Watanabe I (1986) Fundamental studies on environments in plant tissue culture vessels. 2. Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J Agric Meteorol 42:119–127

    Google Scholar 

  • Laforge F, Desjardins Y, Gosselin A (1988) Effets à court et à long termes de l'enrichissement carboné et de la lumière supplémentaire sur l'acclimatation de plants de framboisiers cultivés in vitro. Ann ACFAS 56:196

    Google Scholar 

  • Laforge F, Desjardins Y, Graham MED, Gosselin A (1990) Miniature growth chambers for the study of environmental conditions in vitro. Can J Plant Sci 70:825–836

    Google Scholar 

  • Lee N, Wetzstein HY (1988) Quantum flux density effects on the anatomy and surface morphology of in vitroand in-vivo-developed sweetgum leaves. J Am Soc Hortic Sci 113:167–171

    Google Scholar 

  • Markhart AH, Lin T-Y (1985) New hand-operated press for the extraction of tissue sap for the measurement of osmotic potential. Agron J 77:182–185

    Google Scholar 

  • Menge JA, Davis RE, Johnson ELV, Zentmeyer GA (1978) Myorrhizal fungi increase growth and reduce transplant injury in avocado. Calif Agric 32:6–10

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Nelsen CE (1987) The water relations of vesicular-arbuscular mycorrhizal system. In: Saif GR (ed) Ecophysiology of VA mycorrhizal plants. CRC Press, Boca Raton, Fla, pp 71–91

    Google Scholar 

  • Pierik RLM (1987) Handicaps for the large-scale commercial application of micropropagation. Acta Hortic 230:63–71

    Google Scholar 

  • Pierik RLM (1988) In vitro culture of higher plants as a tool in propagation of horticultural crops. Acta Hortic 226:25–40

    Google Scholar 

  • Ponton F, Piché Y, Parent S, Caron M (1990) The use of vesicular-arbuscular mycorrhizal in boston fern production. I. Effects of peat-based mixes. HortScience 25:183–189

    Google Scholar 

  • Safir GR, Boyer JS, Gerdeman JW (1972) Nutrient status and mycorrhizal enhancement of water transport in soybean. Plant Physiol 49:700–703

    Google Scholar 

  • SAS Institute Inc (1982) SAS user's guide. Statistics. SAS Institute, Cary, NC

    Google Scholar 

  • Shubert A, Bodrino C, Gribaudo I (1992) Vesicular-arbuscular mycorrhizal inoculation of kiwi fruit (Actinidia deliciosa) micropropagated plants. Agronomie 12:847–850

    Google Scholar 

  • Strullu DG (1985) Les mycorhizes. Handbuch der Pflanzen-Anatomie. Portraeger, Berlin

    Google Scholar 

  • Vestberg M (1992) Arbuscular mycorrhizal inoculation of micropropagated strawberry and field observations in Finland. Agronomie 12:865–867

    Google Scholar 

  • Wang H, Parent S, Gosselin A, Desjardins Y (1993) Study of vesicular-arbuscular mycorrhizal peat-based substrates on symbiosis establishment, acclimatization and growth of three micropropagated species. J Am Soc Hortic Sci 118:896–901

    Google Scholar 

  • Wetzstein HY, Sommer HE (1982) Leaf anatomy of tissue-cultured Liquidambar styraciflua plantlets during acclimatization. J Am Soc Hortic Sci 108:475–480

    Google Scholar 

  • Williams SCK, Vestberg M, Uosukain M, Dodd JC, Jeffries P (1992) Effect of fertilizers and arbuscular mycorrhizal fungi on the post-vitro growth of micropropagated strawberry. Agronomie 12:851–857

    Google Scholar 

Download references

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Authors and Affiliations

  1. Centre de Recherche en Horticulture, Faculté des Sciences de l'Agriculture et de l'Alimentation,Université Lavai, G1K 7P4, Québec, Canada

    Abdelmalek Elmeskaoui & Yves Desjardins

  2. Centre de Recherche en Biologie Forestière Faculté de Foresterie et de Géomatique, Université Laval, G1K 7P4, Québec, Canada

    Jean-Pierre Damont, Marie-Josée Poulin & Yves Piché

Authors
  1. Abdelmalek Elmeskaoui
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  2. Jean-Pierre Damont
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  3. Marie-Josée Poulin
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  4. Yves Piché
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  5. Yves Desjardins
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Elmeskaoui, A., Damont, JP., Poulin, MJ. et al. A tripartite culture system for endomycorrhizal inoculation of micropropagated strawberry plantlets in vitro. Mycorrhiza 5, 313–319 (1995). https://doi.org/10.1007/BF00207403

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