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Zum polymorphismus der alkalischen phosphatase (EC3.1.3.1.)

Polymorphism in alkaline phosphatase

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Summary

1. In horizontal starch-gel electrophoresis placental extracts show an alkaline phosphatase polymorphism which — according to ROBSON and HARRIS — depends on the fetal genotype. Investigations carried out with a series of 73 placentas revealed 5 of the common 6 phenotypes. Their frequencies were in good accordance with the figures published by the English authors. However, there had been difficulties in typing because of obscure realizing of the patterns.

2. Although there exists a polymorphism of the heat-stable alkaline phosphatase as observed in 66 serum samples from women at delivery, only four types could be distinguished which, however, could not be related to the placental patterns with any certainty.

3. In all but one of 66 samples of cord serum, investigated simultaneously, a single band only could be observed moving at a slower speed than the serum bands of adult pregnant and non-pregnant persons. This is in conformity with the results of the other investigators.

4. In 18 samples of unheated serum the amount of heat-labile alkaline phosphatase was unimportant and did not alter the features of the heat-stable portion of the enzyme.

5. Contrary to expectations, the alkaline phosphatase polymorphism has no medicolegal relevance.

Zusammenfassung

1. Plazenta-Extrakte weisen in der horizontalen Stärkegel-Elektrophorese einen Polymorphismus der alkalischen Phosphatase auf, der nach ROBSON und HARRIS vom Genotyp des Feten bestimmt wird. In 73 untersuchten Plazentaproben wurden 5 der 6 häufigen Phänotypen beobachtet, deren Frequenz sehr gute Übereinstimmung mit den Werten der englischen Autoren aufwies. Die Typisierung war allerdings wegen undeutlicher Darstellung der Muster sehr schwierig.

2.In 66 Seren von Müttern wurde zum Geburtstermin zwar auch ein Polymorphismus der hitzestabilen alkalischen Phosphatase beobachtet, der jedoch nur 4 Typen umfaßte und keine sichere Zuordnung zu den Plazentamustern gestattete.

3. Die 66 untersuchten Nabelschnurseren wiesen in Übereinstimmung mit anderen Autoren bis auf 1 Ausnahme nur 1 Bande auf, die langsamer wanderte als die Serum-Fraktionen der erwachsenen schwangeren und nichtschwangeren Probanden.

4. Hitzelabile alkalische Phosphatase war bei 18 Seren, die auch ohne Erhitzen untersucht wurden, nur gering konzentriert und störte die Darstellung des hitzestabilen Enzyms nicht.

5. Entgegen den Erwartungen hat der Polymorphismus der hitzestabilen alkalischen Phosphatase keine rechtsmedizinische Relevanz.

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  1. Institut für Rechtsmedizin der Universität Marburg, BRD

    I. Oepen & F. Müller

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  1. I. Oepen
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  2. F. Müller
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Oepen, I., Müller, F. Zum polymorphismus der alkalischen phosphatase (EC3.1.3.1.). Z Rechtsmed 77, 299–309 (1976). https://doi.org/10.1007/BF00203832

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