Abstract
A micropropagation protocol of parsley,Petroselinum crispum (Mill.) Nyman (curled type) has been developed. Surface-sterilized axillary buds cultured on Murashige and Skoog medium supplemented with benzyladenine, kinetin, or thidiazuron developed axillary shoots (rosettes). Kinetin resulted in only a low proliferation rate. The concentrations of thidiazuron or benzyladenine that were optimal for shoot proliferation, resulted in shoots with a low capability to root. During the rooting treatment, these shoots showed wilting signs. Rooting was increased significantly by using a two-week inductive stage with 2.5 μM naphthaleneacetic acid directly followed by acclimatization. Two proliferation media (5 μM benzyladenine and 0.5 μM naphthaleneacetic acid or 5 μM kinetin and 2.5 μM naphthaleneacetic acid) resulted in moderate proliferation but produced shoots that were easy-to-root. These media have been tested by repeated axillary proliferation on the same medium. The medium with 5 μM benzyladenine and 0.5 μM naphthaleneacetic acid was optimal.
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Abbreviations
- BA:
-
6-Benzyladenine
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige & Skoog
- NAA:
-
α-Naphthaleneacetic acid
References
De Klerk GJ (1990) How to measure somaclonal variation. Acta Bot. Neerl. 39: 129–144
Dostal J & Skladal V (1979) Klonovani KvetakuBrassica oleracea L. var.botrytis L.)in vitro. Sbornik Uvtiz-Zahradnictvi 5–6: 177–185
Masuda K, Koda Y & Okazawa Y (1977) Callus formation and embryogenesis of endosperm tissues of parsley seed cultured on hormone-free medium. Physiol. Plant. 41: 135–138
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Porter NG (1989) Composition and yield of commerical essential oils from parsley. 1: herb oil and crop development. Flavour and Fragrance Journal 4: 207–219
Vandemoortele JL, Billard JP, Boucaud J & Gaspar Th (1994) Régénération de plantules à partir de cals issus de pétioles dePetroselinum crispum (Mill.) Nyman. Med. Fac Landbouww. Univ. Gent. 59/3b: 1455–1459
Vasil IK & Hildebrandt AC (1966) Variations of morphogenetic behaviour in plant tissue cultures II.Petroselinum hortense. Amer. J. Bot. 53: 869–874
Watin C & Bigot C (1989) Régénération de plantes fertiles à partir de cals embryogènes de persil commun (Petroselinum hortense Hoffm.). C.R. Acad. Sci. série III 309: 653–659
Zheng GQ, Kenney PM & Lam LKT (1992) Myristicin: a potential cancer chemoprotective agent from parsley leaf oil. J. Agric. Food Chem. 40: 107–110
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Vandemoortele, J.L., Billard, J.P., Boucaud, J. et al. Micropropagation of parsley through axillary shoot proliferation. Plant Cell Tiss Organ Cult 44, 25–30 (1996). https://doi.org/10.1007/BF00045909
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DOI: https://doi.org/10.1007/BF00045909