Abstract
A cDNA encoding an acyl-CoA-binding protein (ACBP) homologue has been cloned from a λgt11 library made from mRNA isolated from developing seeds of oilseed rape (Brassica napus L.). The derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with ACBP sequences from yeast, cow, man and fruit fly. Southern blot analysis ofBrassica napus genomic DNA revealed the presence of 6 genes, 3 derived from theBrassica rapa parent and 3 fromBrassica oleracea. Northern blot analysis showed that ACBP genes are expressed strongly in developing embryo, flowers and cotyledons of seedlings and to a lesser extent in leaves and roots.
References
Keddie JS, Hübner G, Slocombe SP, Jarvis RP, Cummins I, Edwards E-W, Shaw CH, Murphy DJ: Cloning and characterization of an oleosin gene fromBrassica napus. Plant Mol Biol 19: 443–453 (1992).
Kozak M: The scanning model for translation: an update. J Cell Biol 108: 229–235 (1989).
Kragelund BB, Andersen KV, Madsen JC, Knudsen J, Poulsen FM: Three-dimensional structure of the complex between acyl-coenzyme A binding protein and palmitoyl-coenzyme A. J Mol Biol 230: 1260–1277 (1993).
Lagueux M, Bulet P, Hetru C: Diazepam binding inhibitor/endozepine/acyl-CoA-binding homologue fromDrosophila melanogaster. EMBL database, accession number X75596 (1993).
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburn L: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181 (1987).
Lydiate D, Sharpe A, Lagercrantz U, Parkin I: Mapping theBrassica genome. Outlook Agric 22: 85–92 (1993).
Marquardt H, Todaro GJ, Shoyab M: Complete amino acid sequences of bovine and human endozepines. J Biol Chem 261: 9727–9731 (1986).
Murphy DJ, Keen JN, O'Sullivan JN, Au DMY, Edwards EW, Jackson PJ, Cummins I, Gibbons T, Shaw CH, Ryan AJ: A class of amphipathic proteins associated with lipid storage bodies in plants. Possible similarities with animal serum apolipoproteins. Biochim Biophys Acta 1088: 86–94 (1991).
Prescott A, Martin C: A rapid method for the quantitative assessment of levels of specific mRNAs in plants. Plant Mol Biol Rep 4: 219–224 (1987).
Rasmussen JT, Rosendal J, Knudsen J: Interaction of acyl-CoA binding protein (ACBP) on processes for which acyl-CoA is a substrate, product or inhibitor. Biochem J 292: 907–913 (1993).
Rose TM, Schultz ER, Todaro GJ: Molecular cloning of the gene for the yeast homolog (ACB) of diazepam binding inhibitor/endozepine/acyl-CoA-binding protein. Proc Natl Acad Sci USA 89: 11287–11291 (1992).
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Hills, M.J., Dann, R., Lydiate, D. et al. Molecular cloning of a cDNA fromBrassica napus L. for a homologue of acyl-CoA-binding protein. Plant Mol Biol 25, 917–920 (1994). https://doi.org/10.1007/BF00028886
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DOI: https://doi.org/10.1007/BF00028886