Abstract
An overview is provided of the results of biochemical investigations performed on the rotifer Brachionus plicatilis. Hydrolytic enzymes (mostly glycosidases and proteinases) from Brachionus plicatilis were examined in total homogenates and in fractions obtained by differential and by density gradient centrifugations. By this means (1) soluble, (2) membrane associated and (3) lysosomal enzymes could be distinguished. The first two classes are presumed to be involved in extracellular digestion processes. Integument material from Brachionus was prepared by dissolving the rotifer tissues with strong protein denaturants which leave the material of the intrasyncytial dense lamina, or lorica, intact. The lorica material was then dissolved by treatment with denaturants plus disulphide breaking agents, and characterized by electrophoretic methods, by its sensitivity to proteinases and by its cross-reactivity with keratine-antibodies. Application of these methods to other rotifer species has revealed that at least two types of integument-material with differing chemical properties occur in rotifers. Using similar methods trophi-material of the mastax was prepared for morphological as well as chemical examination. These investigations showed that the trophi contain chitin.
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Bartnik, E., M. Osborn & K. Weber, 1986. Intermediate filaments in non-neuronal cells of invertebrates: isolation and biochemical characterization of intermediate filaments from the esophageal epithelium of the mollusc Helix pomatia. J. Cell. Biol. 101: 427–440.
Bartnik, E. & K. Weber, 1989. Widespread occurrence of intermediate filaments in invertebrates; common principles and aspects of diversion. Eur. J. Cell. Biol. 50: 17–33.
Bender, K. & W. Kleinow, 1988. Chemical properties of the lorica and related parts from the integument of Brachionus plicatilis. Comp. Biochem. Physiol. 89B: 483–487.
Clément, P., 1969. Premieres observations sur l'ultrastructure comparee des teguments de Rotiferes. Vie Milieu A 20: 461–482.
Depoortere, H. & N. Magis, 1967. Mise en evidence, localisation et dosage de la chitine dans la coque des oefs de Brachionus leydigii Cohn et d'autres rotiferes. Ann. Soc. R. zool. Belgique 97: 187–195.
de Duve, C., 1975. Exploring cells with a centrifuge. Science 189: 186–194.
Hara, K., H. Arano & T. Ishihara, 1984a. Purification of alkaline protease from the rotifer Brachionus plicatilis. Bull. Jap. Soc. Sci. Fish. 50: 1605–1609.
Hara K, H. Arano & T. Ishihara, 1984b. Some enzymatic properties of alkaline proteases of the rotifer Brachionus plicatilis. Bull. Jap. Soc. Sci. Fish. 50: 1611–1616.
Kleinow, W. & K. Kühle, 1984. Zum pH-Optimum hydrolytischer Enzyme und zum pH-Milieu im Verdauungstrakt bei Brachionus plicatilis (Rotatoria). Verh. Dtsch. Zool. Ges. 77: 299.
Kleinow, W. & K. Bender, 1986. Zur chemischen Zusammensetzung des Hautpanzers von Brachionus plicatilis (Rotatoria). Verh. Dtsch. Zool. Ges. 79: 389.
Kleinow, W., C. Wethmar & M. Karisch, 1987. Proteinasen aus Brachionus plicatilis (Rotatoria): Charakterisierung durch gelelektrophoretische Methoden. Verh. Dtsch. Zool. Ges. 80: 286–287.
Kleinow, W. & K. Wißling, 1988. Charakterisierung von Proteinen aus dem Hautpanzer von Brachionus plicatilis (Rotatoria): Hinweise auf keratinartige Komponenten. Verh. Dtsch. Zool. Ges. 81: 310–311.
Kleinow, W. & K. Wißling, 1989. Keratin-like proteins from the integument of the rotifer Brachionus plicatilis. Biol. Chem. Hoppe-Seyler 370: 918–919.
Kleinow, W., J. Klusemann & H. Wratil, 1990. A gentle method for the preparation of hard parts (trophi) of the mastax of rotifers and scanning electron microscopy of the trophi of Brachionus plicatilis (Rotifera). Zoomorphology 109: 329–336.
Kleinow, W., H. Wratil, K. Kühle & B. Esch, 1991. Electron microscope studies of the digestive tract of Brachionus plicatilis (Rotifera). Zoomorphology 111: 67–80.
Klusemann, J., W. Kleinow & W. Peters, 1990. The hard parts of the rotifer mastax do contain chitin: evidence from studies on Brachionus plicatilis. Histochemistry 94: 277–283.
Koehler, J. K., 1965. A fine-structure study of the rotifer integument. J. Ultrastruct. Res. 12: 113–134.
Koehler, J. K. & T. L. Hayes, 1969. The rotifer jaw: a scanning and transmission electron microscope study I. The trophi of Philodina acuticornis odiosa. J. Ultrastruct. Res. 27: 402–418.
Kühle, K., 1987. Untersuchungen zur Verdauungsphysiologie von Brachionus plicatilis (Rotatoria): Feinmorphologie des Verdauungstraktes und Versuche zur Charakterisierung und Lokalisierung von Carbohydrasen. Thesis of doctorate, Universität zu Köln.
Kühle, K. & W. Kleinow, 1985. Measurements of hydrolytic enzymes in homogenates from Brachionus plicatilis (Rotifera). Comp. Biochem. Physiol. 81B: 437–442.
Kühle, K. & W. Kleinow, 1989a. Localization of hydrolytic enzymes within cellular fractions from Brachionus plicatilis (Rotatoria). Comp. Biochem. Physiol. 93B: 565–574.
Kühle, K. & W. Kleinow, 1989b. Zur Feinstruktur der Zellen im Verdauungstrakt von Brachionus plicatilis (Rotatoria) Verh. Dtsch. Zool. Ges. 82: 187.
Kühle, K. & W. Kleinow, 1990. Glycosidases in Brachionus plicatilis (Rotifera). Comp. Biochem. Physiol. 95B: 393–402.
Storch, V. & U. Welsch, 1969. Über den Aufbau des Rotatorieninteguments. Z. Zellforsch. 95: 405–414.
Wurdak, E. S., 1987. Ultrastructure and histochemistry of the stomach of Asplanchna sieboldi. Hydrobiologia 147: 361–371.
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Kleinow, W. Biochemical studies on Brachionus plicatilis: hydrolytic enzymes, integument proteins and composition of trophi. Hydrobiologia 255, 1–12 (1993). https://doi.org/10.1007/BF00025814
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DOI: https://doi.org/10.1007/BF00025814