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Effect of flooding on the activities of some enzymes of activated oxygen metabolism, the levels of antioxidants, and lipid peroxidation in senescing tobacco leaves

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Abstract

Effects of flooding on the activities of some enzymes of activated oxygen metabolism, the levels of antioxidants, and lipid peroxidation in senescing leaves of tobacco were investigated. As judged by the decrease in chlorophyll and protein levels, flooding accelerated the senescence of tobacco leaves. Total peroxide and the lipid peroxidation product, malondialdehyde, increased in both control and flooding-treated leaves with increasing duration of the experiment. Throughout the duration of the experiment, flooded leaves had higher levels of total peroxide and malondialdehyde than did control leaves. Flooding resulted in an increase in peroxidase and ascorbate peroxidase activities and a reduction of superoxide dismutase activity in the senescing leaves. Glycolate oxidase, catalase, and glutathione reductase activities were not affected by flooding. Flooding increased the levels of total ascorbate and dehydroascorbate. Total glutathione, reduced form glutathione, or oxidized glutathione levels in flooded leaves were lower than in control leaves during the first two days of the experiment, but were higher than in control leaves at the later stage of the experiment. Our work suggests that senescence of tobacco induced by flooding may be a consequence of lipid peroxidation possibly controlled by superoxide dismutase activity. Our results also suggest that increased rates of hydrogen peroxide in leaves of flooded plants could lead to increased capacities of the scavenging system of hydrogen peroxide.

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Abbreviations

GSH:

reduced form glutathione

GSSG:

oxidized form glutathione

GSSG reductase:

glutathione reductase

MDA:

malondialdehyde

SOD:

superoxide dismutase

References

  1. Brennan T, Rychter A, Frenkel C (1979) Activity of enzymes involved in the turnover of hydrogen peroxide during fruit senescence. Bot Gaz 140: 384–388

    Article  Google Scholar 

  2. Chowdhury SR and Choudhuri MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiol Plant 65: 503–507

    Google Scholar 

  3. Curtis CR (1971) Disc electrophoretic comparisons of proteins and peroxidases from Phaseolus vulgaris leaves infected with Agrobacterium tumefaciens. Can J Bot 49: 333–337

    Google Scholar 

  4. Dhindsa RS and Reid DM (1982) Leaf senescence and lipid peroxidation: Effects of some phytohormones, and scavengers of free radicals and singlet oxygen. Physiol Plant 56: 453–457

    Google Scholar 

  5. Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 126: 93–101

    Google Scholar 

  6. Elstner EF (1982) Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 33: 73–96

    Article  Google Scholar 

  7. Foster JG and Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66: 482–487

    Google Scholar 

  8. Gillham DJ and Dodge AD (1984) Some effects of paraquat on plants. Plant Physiol 75: S-51

    Google Scholar 

  9. Goyal A (1987) Effect of water stress on glycolate metabolism in the leaves of rice seedlings (Oryza sativa). Physiol Plant 69: 289–294

    Google Scholar 

  10. Gross GG, Jansh C, Elstner EF (1977) Involvement of malate, monophenols, and the superoxide radical in hydrogen peroxide formation by isolated cell walls from horseradish (Amoracia lapathifolia Gilib.). Planta 136: 271–276

    Google Scholar 

  11. Heath RL and Packer L (1968) Photoperoxidation in isolated chloroplasts. 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189–198

    PubMed  Google Scholar 

  12. Hunter MIS, Hetherington AM, Crawford RMM (1983) Lipid peroxidation — a factor in anoxia intolerance in Iris species? Phytochemistry 22: 1145–1147

    Article  Google Scholar 

  13. Hurng WP and Kao CH (1993) Growth response of tobacco to flooding. Bot Bull Acad Sin 34 (In press)

  14. Kato M and Shimuzu S (1987) Chlorophyll metabolism in higher plants VII. Chlorophyll degradation in senescring tobacco leaves: phenolic-dependent peroxidative degradation. Can J Bot 65: 729–735

    Google Scholar 

  15. Kellogg EW and Fridovich I (1975) Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J Biol Chem 250: 8812–8817

    PubMed  Google Scholar 

  16. Kozlowski TT (1984) Plant responses to flooding of soil. BioScience 34: 162–167

    Google Scholar 

  17. Laws MY, Charles SA, Halliwell B (1983) Glutathione and ascorbic acid in spinach chloroplasts: the effect of hydrogen peroxide and of paraquat. Biochem J 210: 899–903

    PubMed  Google Scholar 

  18. Leshem YY (1981) Oxy free radicals and plant senescence. What's New Plant Physiol 12: 1–4

    Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275

    PubMed  Google Scholar 

  20. Mondal R and Choudhuri MA (1981) Role of hydrogen peroxide in senescence of excised leaves of rice and maize. Biochem Physiol Pflanz 176: 700–709

    Google Scholar 

  21. Mondal R and Choudhuri MA (1982) Regulation of senescence of excised leaves of some C3 and C4 species by endogenous H2O2. Biochem Physiol Pflanz 177: 403–417

    Google Scholar 

  22. Mondal R and Choudhuri MA (1984) Interaction of phytohormones with H2O2 metabolism and senescence of excised leaves of some C3 and C4 plants. Biochem Physiol Pflanz 179: 463–471

    Google Scholar 

  23. Monk LS, Fagerstedt KV, Crawford RMM (1989) Oxygen toxicity and superoxide dismutase as an antioxidant in physiological stress. Physiol Plant 76: 456–459

    Google Scholar 

  24. Mukherjee SP and Choudhuri MA (1983) Implication of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58: 166–170

    Google Scholar 

  25. Nakano Y and Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–880

    Google Scholar 

  26. Parida RK, Kar M, Mishra D (1978) Enhancement of senescence in excised rice leaves by hydrogen peroxide. Can J Bot 56: 2937–2941

    Google Scholar 

  27. Pauls KR and Thompson JE (1984) Evidence for the accumulation of peroxidized lipids in membranes of senescing cotyledons. Plant Physiol 75: 1152–1157

    Google Scholar 

  28. Pedersen TC and Aust SD (1973) The role of superoxide and singlet oxygen in lipid peroxidation promoted by xanthine oxidase. Biochem Biophys Res Commun 52: 1071–1078

    PubMed  Google Scholar 

  29. Polle A, Krings B, Ronnenberg H (1989) Superoxide dismutase activity in needles of Norwegian spruce tree (Picea abies L.). Plant Physiol 90: 1310–1315

    Google Scholar 

  30. Price ML and Butler LG (1977) Rapid visual estimation and spectrometric determination of tannin content of sorghum grain. J Agric Food Chem 25: 1268–1273

    Google Scholar 

  31. Sagisaka S (1976) The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol 57: 308–309

    Google Scholar 

  32. Shigeoka S, Onishi T, Nakano Y, Kitaoka S (1987) Photo-induced biosynthesis of glutathione in Euglena. Agric Biol Chem 43: 2053–2058

    Google Scholar 

  33. Shigeoka S, Yokota A, Nakano Y and Kitaoka S (1979) The effect of illumination on the L-ascorbic acid in Euglena gracilis Z. Agric Biol Chem 43: 2053–2058

    Google Scholar 

  34. Smirnoff N and Colombe SV (1988) Drought influences the activity of enzymes of the chloroplast hydrogen peroxide scavenging system, J Exp Bot 39: 1097–1108

    Google Scholar 

  35. Smith IK (1985) Stimulation of glutathione synthesis in photorespiring plants by catalase inhibitors. Plant Physiol 79: 1044–1047

    Google Scholar 

  36. Tanaka K, Suda Y, Kondo N, Sugahara K (1985) O3 tolerance and the ascorbate-dependent H2O2 decomposing system in chloroplasts. Plant Cell Physiol 26: 1425–1431

    Google Scholar 

  37. Thomas H and Stoddart JL (1981) Leaf senescence. Annu Rev Plant Physiol 31: 83–111

    Article  Google Scholar 

  38. Ushimaru T, Shibasaka M, Tsujii H (1992) Development of the O2-detoxification system during adaptation to air of submerged rice seedlings. Plant Cell Physiol 33: 1065–1071

    Google Scholar 

  39. Van Toai TT and Bolles CS (1991) Postanoxic injury in soybean (Glycine max) seedlings. Plant Physiol 97: 588–592

    Google Scholar 

  40. Wintermans JFGM and De Mots A (1965) Spectrophotometric characteristic of chlorophylls a and b and their pheophytins in ethanol. Biochim Biophys Acta 109: 448–453

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Taiwan, Republic of China

    Weei Pirng Hurng & Ching Huei Kao

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  1. Weei Pirng Hurng
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  2. Ching Huei Kao
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Hurng, W.P., Kao, C.H. Effect of flooding on the activities of some enzymes of activated oxygen metabolism, the levels of antioxidants, and lipid peroxidation in senescing tobacco leaves. Plant Growth Regul 14, 37–44 (1994). https://doi.org/10.1007/BF00024139

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  • DOI: https://doi.org/10.1007/BF00024139

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