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Odorant Binding Proteins as Sensing Layers for Novel Gas Biosensors: An Impedance Spectroscopy Characterization

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Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 91))

Abstract

In this work, an ab-initio study of the electrical response to odorants of a self-assembled monolayer of a pig OBP immobilized onto a miniaturized Si-substrate equipped with gold interdigitated electrodes (IDE), was started. Electrical Impedance Spectroscopy (EIS) was used as electrical characterization technique and a dedicated experimental set-up was arranged in order to carry out EIS measurements in controlled environment. The EIS data was fitted by using a fitting software based on Levenberg–Marquardt (LEVM) algorithm to determine the equivalent circuit of the system.

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Acknowledgment

We are grateful to GOSPEL (FP6-IST 507610) for financing this initial study, and to Prof. Paolo Pelosi, University of Pisa for the Pig Odorant Binding Proteins.

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Correspondence to S. Capone .

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Capone, S., De Pascali, C., Francioso, L., Siciliano, P., Persaud, K.C., Pisanelli, A.M. (2011). Odorant Binding Proteins as Sensing Layers for Novel Gas Biosensors: An Impedance Spectroscopy Characterization. In: Neri, G., Donato, N., d'Amico, A., Di Natale, C. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 91. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1324-6_49

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  • DOI: https://doi.org/10.1007/978-94-007-1324-6_49

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  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-007-1323-9

  • Online ISBN: 978-94-007-1324-6

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