Abstract
A breakthrough in ultrathin sectioning was the introduction of diamond knives [1]. Glass knives are suitable for the sectioning of acrylate or epoxy resin embedded biological samples. However, diamond knives allow the sectioning of a great variety of samples. No matter whether these samples are soft and hard (of biological or of materials origin), or whether they are sectioned at room or at low temperatures. Diamond knives are used for wet and dry sectioning (the latter eg is mandatory for secondary ion mass spectroscopy SIMS [2]). Furthermore diamond knives are long lasting and very sharp-egded. The radius curvature of high quality diamond cutting edges is less than 5nm [3].
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Gnaegi, H., Studer, D., Bos, E., Peters, P., Pierson, J. (2008). Ultramicrotomy in biology and materials science: an overview. In: Luysberg, M., Tillmann, K., Weirich, T. (eds) EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85156-1_399
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DOI: https://doi.org/10.1007/978-3-540-85156-1_399
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