Abstract:
Yarrowia lipolytica is one of the most important non-conventional oleaginous yeasts. This review summarizes the recent advances in the taxonomy, physiology, growth requirements and environmental distribution of this particular yeast. It provides as well an extensive summary of its genomic features. Due to the remarkable potential to accumulate and modify lipids and the ability to grow on various hydrophobic substrates, Y. lipolytica has been now seriously considered as a bioconversion model for production of single-cell proteins, enzymes and lipid-derived compounds. Its presence in a variety of environments polluted with petroleum and in particular with its heavy fractions, witnesses its pivotal role in degradation of recalcitrant organic compounds. Some of them (naphthalene, dibenzofuran, and trinitrotoluene) have been already successfully tested and the process has proved to be functional. Y. lipolytica is also found in a variety of marine and hypersaline environments, thus the use of this yeast as a model system for salt-stress studies could be envisaged. Newly developed genetic tools and available genome sequence data will allow more complete exploration of this yeast in the areas of biocatalysis, bioconversion, and bioremediation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Andreishcheva EN, Isakova EP, Sidorov NN, Abramova NB (1999) Adaptation to salt stress in a salt-tolerant strain of the yeast Yarrowia lipolytica. Biochemistry (Mosc) 64: 1061–1067.
Athenstaedt K, Jolivet P, Boulard C, Zivy M, Negroni L, Nicaud JM, Chardot T (2006) Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source. Proteomics 6: 1450–1459.
Atlas RM (1992) Molecular methods for environmental monitoring and containment of genetically engineered microorganisms. Biodegradation 3: 137–146.
Barnett JA, Payne RW, Yarrow D (1983) Yeasts: characteristics and identification. Cambridge: Cambridge University Press.
Barth G, Gaillardin C (1996) Yarrowia lipolytica. In Nonconventional Yeasts in Biotechnology: A Handbook. K Wolf (ed.). Berlin: Springer, pp. 313–388.
Butinar SS, Spencer-Martins I, Oren A, Gunde-Cimerman N (2005) Yeast diversity in hypersaline habitats. FEMS Microbiol Lett 244: 229–234.
Casaregola S, Feynerol C, Diez M, Fournier P, Gaillardin C (1997) Genomic organization of the yeast Yarrowia lipolytica. Chromosoma 106: 380–390.
Cerniglia CE, Crow SA (1981) Metabolism of aromatic hydrocarbons by yeasts. Arch Microbiol 129: 9–13.
Chi Z, Wang F, Wang L, Li J, Wang X (2007) Selection of Yarrowia lipolytica strains with high protein content from yeasts isolated from different marine environments. J Ocean University of China 6: 360–364.
Cholet O, Henaut A, Casaregola S, Bonnarme P (2007) Gene expression and biochemical analysis of cheese-ripening yeasts: focus on catabolism of L-methionine, lactate, and lactose. Appl Environ Microbiol 73: 2561–2570.
Desai JD, Banat IM (1997) Microbial production of surfactants and their commercial potential. Microbiol Mol Biol Rev 61: 47–64.
De Felice B, Pontecorvo G, Carfagna M (1997) Degradation of waste waters from olive oil mills by Yarrowia lipolytica ATCC 20225 and Pseudomonas putida. Acta Biotechnol 17: 231–239.
Dominguez A, Costas M, Longo MA, Sanroman A (2003) A novel application of solid state culture: production of lipases by Yarrowia lipolytica. Biotechnol Lett 25: 1225–1229.
Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, de Montigny J, Marck C, Neuvéglise C, Talla E, Goffard N, Frangeul L, Aigle M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich J-M, Beyne E, Bleykasten C, Boisrame A, Boyer J, Cattolico L, Confanioleri F, de Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, Groppi A, Hantraye F, Hennequin C, Jauniaux N, Joyet P, Kachouri R, Kerrest A, Koszul R, Lemaire M, Lesur I, Ma L, Muller H, Nicaud J-M, Nikolski M, Oztas S, Ozier-Kalogeropoulos O, Pellenz S, Potier S, Richard G-F, Straub M-L, Suleau A, Swennen D, Tekaia F, Wesolowski-Louvel M, Westhof E, Wirth B, Zeniou-Meyer M, Zivanovic I, Bolotin-Fukuhara M, Thierry A, Bouchier C, Caudron B, Scarpelli C, Gaillardin C, Weissenbach J, Wincker P, Souciet J-L (2004) Genome evolution in yeasts. Nature 430: 35–44.
Fickers P, Benetti PH, Wache Y, Marty A, Mauersberger S, Smit MS, Nicaud JM (2005) Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Res 5: 527–543.
Fickers P, Le Dall MT, Gaillardin C, Thonart P, Nicaud JM (2003) New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica. J Microbiol Methods 55: 727–737.
Gao L, Chi Z, Sheng J, Wang L, Li J, Gong F (2007). Inulinase-producing marine yeasts: evaluation of their diversity and inulin hydrolysis by their crude enzymes. Microb Ecol 54: 722–729.
Gutierrez JR, Erickson LE (1977) Hydrocarbon uptake in hydrocarbon fermentation. Biotechnol Bioeng 19: 1331–1349.
Harris JC, Steinmetz EA (1967) Emulsified jet engine fuel. JAE Technical Report, No. 670365.
Imada A, Sinskey AJ, Tannenbaum SR (1972) Degradation of ribonucleic acid in Candida lipolytica. Extraction of ribonucleic acid degrading enzymes J Biotechnol Bioeng 14: 103–122.
Jain MR, Zinjarde SS, Deobagkar DD, Deobagkar DN (2004) 2,4,6-Trinitrotoluene transformation by a tropical marine yeast, Yarrowia lipolytica NCIM 3589. Marine Poll Bull 49: 783–788.
Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30: 3059–66.
Kim JT, Kang SG, Woo JH, Lee JH, Jeong BC, Kim SJ (2007) Screening and its potential application of lipolytic activity from a marine environment: characterization of a novel esterase from Yarrowia lipolytica CL180. Appl Microbiol Biotechnol 74: 820–828.
King RB, Long GM, Sheldon JK (1992) Practical Environmental Bioremediation. Boca Raton: Lewis Publishers.
Madzak C, Gaillardin C, Beckerich J-M (2004) Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. Journal of Biotechnology 109: 63–81.
Mauersberger S, Wang HJ, Gaillardin C, Barth G, Nicaud JM (2001) Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genes involved in hydrophobic substrate utilization. J Bact 183: 5102–5109.
Mauersberger S, Nicaud J-M (2003) Tagging genes by insertional mutagenesis in the yeast Yarrowia lipolytica. In Laboratory Manual on Non-conventional Yeast in Genetics, Biochemistry and Biotechnology. K Wolf, K Breunig, and G Barth (eds.). Berlin-Heidelberg-New York: Springer, pp. 343–356.
Morin M, Monteoliva L, Insenser M, Gil C, Dominguez A (2007) Proteomic analysis reveals metabolic changes during yeast to hypha transition in Yarrowia lipolytica. J Mass Spectrom 42: 1453–1462.
Neuvéglise C, Bon E, Lepingle A, Wincker P, Artiguenave F, Gaillardin C, Casaregola S (2000) Genomic exploration of the hemiascomycetous yeasts: 9. Saccharomyces kluyveri. FEBS Lett 487: 56–60.
Neuvéglise C, Chalvet F, Wincker P, Gaillardin C, Casaregola S (2005) Mutator-like element in the yeast Yarrowia lipolytica displays multiple alternative splicings. Eukaryot Cell 4: 615–624.
Ogrydziak DM, Mortimer RK (1977) Genetics of extracellular protease production in Saccharomycopsis lipolytica. Genetics 87: 621–632.
Oswal N, Sharma PM, Zinjarde SS, Pant A (2002) Palm oil mill effluent treatment by a tropical marine yeast. Biores Technol 85: 35–37.
Ota Y, Oikawa S, Morimoto Y, Minoda Y (1984) Nutritional factors causing mycelial development of Saccharomycopsis lipolytica. Agric Biol Chem 48: 1933–1940.
Papanikolaou S, Galiotou-Panayotou M, Fakas S, Komaitis M, Aggelis G (2008) Citric acid production by Yarrowia lipolytica cultivated on olive-mill wastewater-based media. Bioresour Technol 99: 2419–2428.
Ratledge C (1994) Yeasts, moulds, algae and bacteria as sources of lipids. In Technological Advances in Improved and Alternative Sources of Lipids. BS Kamel and Y Kakuda (eds.). London: Blackie Academic and Professional, pp. 235–291.
Ratledge C (2005) Single cell oils for the 21st century. In Single Cell Oils. Z Cohen and C Ratlege (eds.). Champaign: AOCS Press, pp. 1–20.
Richard GF, Kerrest A, Lafontaine I, Dujon B (2005) Comparative genomics of hemiascomycete yeasts: genes involved in DNA replication, repair, and recombination. Mol Biol Evol 22: 1011–1023.
Romero MC, Hammer E, Cazau MC, Arambarri AM (2002) Isolation and characterization of biarylic structure-degrading yeasts: hydroxylation potential of dibenzofuran. Environ Pollut 118: 379–382.
Ron EZ, Rosenberg E (2002) Biosurfactants and oil bioremediation. Curr Opin Biotechnol 13: 249–252.
Rosenberg E, Ron EZ (1999) High- and low-molecular-mass microbial surfactants. Appl Microbiol Biotechnol 52: 154–162.
Scioli C, Vollaro L (1997) The use of Yarrowia lipolytica to reduce pollution in olive mill wastewaters. Wat Res 31: 2520–2524.
Swennen D, Beckerich JM (2007) Yarrowia lipolytica vesicle-mediated protein transport pathways. BMC Evol Biol 7: 219–238.
Thevenieau F, Le Dall MT, Nthangeni B, Mauersberger S, Marchal R, Nicaud JM (2007) Characterization of Yarrowia lipolytica mutants affected in hydrophobic substrate utilization. Fungal Genet Biol 44: 531–542.
Thevenieau F, Gaillardin C, Nicaud J-M (2008) Applications of the non-conventional yeast Yarrowia lipolytica. In Diversity and Potential Biotechnological Applications of Yeasts. G Kunze and T Satyanarayana (eds.). Amsterdam: Elsevier.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–82.
Van der Walt JP, von Arx JA (1980) The yeast genus Yarrowia gen. Antonie Van Leeuwenhoek 46: 517–521.
Van Heerikhuizen H, Ykema A, Klootwijk J, Gaillardin C, Ballas C, Fournier P (1985) Heterogeneity in the ribosomal RNA genes of the yeast Yarrowia lipolytica; cloning and analysis of two size classes of repeats. Gene 39: 213–222.
Vergassola M, Vespignani A, Dujon B (2005) Cooperative evolution in protein complexes of yeast from comparative analyses of its interaction network. Proteomics 5: 3116–3119.
Wang L, Chi Z, Wang X, Liu Z, Li J (2007) Diversity of lipase-producing yeasts from marine environments and oil hydrolysis by their crude enzymes. Annals of Microbiol 57: 495–501.
Wickerham LJ, Kurtzman CP, Herman AI (1970) Sexual reproduction in Candida lipolytica. Science 167: 1141–1147.
Wolfe KH, Shields DC (1997) Molecular evidence for an ancient duplication of the entire yeast genome. Nature 387: 708–713.
Yarrow D (1972) Four new combinations in yeasts. Antonie Van Leeuwenhoek 38: 357–360.
Yorifugi T, Jacques S, Sinskey AJ (1975) N-alkane oxidizing enzymes in Candida lipolytica Abstr Journal ASM Annual Meeting p. 171.
Yue L, Chi Z, Wang L, Liu J, Madzak C, Li J, Wang X (2008) Construction of a new plasmid for surface display on cells of Yarrowia lipolytica. J Microbiol Methods 72: 116–123.
Zaripov SA, Naumov AV, Abdrakhmanova JF, Garusov AV, Naumova RP (2002) Models of 2,4,6-trinitrotoluene (TNT) initial conversion by yeasts. FEMS Microbiol Lett 217: 213–217.
Zinjarde SS, Sativel C, Lachke AH, Pant A (1997) Isolation of an emulsifier from Yarrowia lipolytica NCIM 3589 using a modified mini isoelectric focusing unit. Lett in Appl Microbiol 24: 117–121.
Zinjarde SS, Pant A (2000) Crude Oil Degradation by Free and Immobilized Cells of Yarrowia lipolytica NCIM 3589. J Environ Science Health 35: 765–773.
Zinjarde SS, Pant A (2002) Hydrocarbon degraders from a tropical marine environment. Marine Poll Bull 44: 118–121.
Zvyagilskaya R, Andreishcheva E, Soares MI, Khozin I, Berhe A, Persson BL (2001) Isolation and characterization of a novel leaf-inhabiting osmo-, salt-, and alkali-tolerant Yarrowia lipolytica yeast strain. J Basic Microbiol 41: 289–303.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer-Verlag Berlin Heidelberg
About this entry
Cite this entry
Beopoulos, A., Desfougeres, T., Sabirova, J., Zinjarde, S., Neuvéglise, C., Nicaud, JM. (2010). The Hydrocarbon-Degrading Oleaginous Yeast Yarrowia lipolytica . In: Timmis, K.N. (eds) Handbook of Hydrocarbon and Lipid Microbiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77587-4_152
Download citation
DOI: https://doi.org/10.1007/978-3-540-77587-4_152
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-77584-3
Online ISBN: 978-3-540-77587-4
eBook Packages: Biomedical and Life SciencesReference Module Biomedical and Life Sciences