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Pancreatic Islet Isolation

From the Mouse to the Clinic

  • Book
  • © 2016

Overview

Editors:
  1. Miriam Ramírez-Domínguez

    1. Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital Cruces, University of the Basque Country (UPV/EHU), Leioa, Spain

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  • Vivid and colourful illustrations
  • Presents state-of-the-art research and recent advances in islet isolation
  • A special chapter on the different and similar features between animal models and human islets
  • Offers a comparative study of the islet isolation procedure in different animal models and humans

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 938)

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Table of contents (7 chapters)

  1. Front Matter

    Pages i-xii
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  2. Historical Background of Pancreatic Islet Isolation

    • Miriam Ramírez-Domínguez
    Pages 1-9

  3. The Different Faces of the Pancreatic Islet

    • Midhat H. Abdulreda, Rayner Rodriguez-Diaz, Over Cabrera, Alejandro Caicedo, Per-Olof Berggren
    Pages 11-24

  4. Isolation of Mouse Pancreatic Islets of Langerhans

    • Miriam Ramírez-Domínguez
    Pages 25-34

  5. Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets

    • Heide Brandhorst, Paul R. V. Johnson, Daniel Brandhorst
    Pages 35-55

  6. Isolation of Pancreatic Islets from Nonhuman Primates

    • Dora M. Berman
    Pages 57-66

  7. Necessities for a Clinical Islet Program

    • Wayne J. Hawthorne
    Pages 67-88

  8. Clinical Islet Isolation

    • Wayne J. Hawthorne, Lindy Williams, Yi Vee Chew
    Pages 89-122

  9. Back Matter

    Pages 123-123
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Keywords

About this book

Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevantspecies (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.

Editors and Affiliations

  • Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital Cruces, University of the Basque Country (UPV/EHU), Leioa, Spain

    Miriam Ramírez-Domínguez

About the editor

Miriam Ramírez-Domínguez, University of the Basque Country, Faculty of Medicine, Laboratory of Cell Therapy of Diabetes, Leioa, Spain

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