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Cell Culture Model to Study Cerebral Aneurysm Biology

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Subarachnoid Hemorrhage

Part of the book series: Acta Neurochirurgica Supplement ((NEUROCHIRURGICA,volume 127))

Abstract

Mechanisms governing cerebral aneurysm (CA) formation, progression, and rupture remain incompletely understood. However, understanding such mechanisms is critical to improving treatment for patients harboring CA. In vitro studies facilitate dissecting molecular mechanisms underlying vascular pathology and allow screening of therapies that can be subsequently explored in vivo. Cerebral vascular smooth muscle cells (VSMC) are an important constituent of the vessel wall, and phenotypic modulation of these cells to a pro-inflammatory, pro-matrix remodeling phenotype appears to be important in CA pathology. We have taken a reductionist approach using cultured cerebral VSMC to further explore CA biology. We describe techniques for culturing cerebral VSMC and outline experimental approaches incorporating these cells to study CA biology.

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Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Neurosurgery, Tulane Center for Clinical Neurosciences, Tulane University School of Medicine, New Orleans, LA, USA

    Alejandra N. Martinez, Crissey L. Pascale, Peter S. Amenta, Rachel Israilevich & Aaron S. Dumont

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  1. Alejandra N. Martinez
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  2. Crissey L. Pascale
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  3. Peter S. Amenta
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  4. Rachel Israilevich
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  5. Aaron S. Dumont
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Corresponding author

Correspondence to Aaron S. Dumont .

Editor information

Editors and Affiliations

  1. Department of Anethesiology, Loma Linda University Medical Center, Loma Linda, CA, USA

    Robert D. Martin

  2. Department of Neurosurgery, Loma Linda University Medical Center, Loma Linda, CA, USA

    Warren Boling

  3. Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou, China

    Gang Chen

  4. Department of Anesthesiology and Physiology, Loma Linda University Medical Center, Loma Linda, CA, USA

    John H. Zhang

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Martinez, A.N., Pascale, C.L., Amenta, P.S., Israilevich, R., Dumont, A.S. (2020). Cell Culture Model to Study Cerebral Aneurysm Biology. In: Martin, R., Boling, W., Chen, G., Zhang, J. (eds) Subarachnoid Hemorrhage. Acta Neurochirurgica Supplement, vol 127. Springer, Cham. https://doi.org/10.1007/978-3-030-04615-6_5

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  • DOI: https://doi.org/10.1007/978-3-030-04615-6_5

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-04614-9

  • Online ISBN: 978-3-030-04615-6

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