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Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection

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Staphylococcus Epidermidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1106))

Abstract

Animal models are invaluable tools for translational research, allowing investigators to recapitulate observed clinical scenarios within the laboratory that share attributes with human disease. Here, we describe a mouse model of post-arthroplasty Staphylococcus epidermidis joint infection which mimics human disease and may be utilized to explore the complex series of events during staphylococcal implant-associated infections by identifying key immunological, bacterial, and/or therapeutic mechanisms relevant to these persistent infections.

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References

  1. Fey PD, Olson ME (2010) Current concepts in biofilm formation of Staphylococcus epidermidis. Future Microbiol 5:917–933

    Article  PubMed  CAS  Google Scholar 

  2. McCann MT, Gilmore BF, Gorman SP (2008) Staphylococcus epidermidis device-related infections: pathogenesis and clinical management. J Pharm Pharmacol 60:1551–1571

    PubMed  CAS  Google Scholar 

  3. Rohde H, Frankenberger S, Zahringer U et al (2010) Structure, function and contribution of polysaccharide intracellular adhesion (PIA) to Staphylococcus epidermidis biofilm formation and pathogenesis of biomaterial-associated infections. Eur J Cell Biol 89:103–111

    Article  PubMed  CAS  Google Scholar 

  4. Baillot R, Frechette E, Cloutier D et al (2012) Surgical site infections following transcatheter apical aortic valve implantation: incidence and management. Eur J Cardio Thorac Surg 7:122

    Google Scholar 

  5. Kane AD, Ndiaye MB, Pessinaba S et al (2012) Infections secondary to pacemaker implantation: a synopsis of six cases. Cardiovasc J Afr 23:e1–e4

    Article  PubMed  CAS  Google Scholar 

  6. Rosenthal ME, Dever LL, Moucha CS et al (2011) Molecular characterization of an early invasive Staphylococcus epidermidis prosthetic joint infection. Microb Drug Resist 17:345–350

    Article  PubMed  Google Scholar 

  7. Esteban J, Sandoval E, Cordero-Ampuero J et al (2012) Sonication of intramedullary nails: clinically-related infection and contamination. Open Orthop J 6:255–260

    Article  PubMed  CAS  Google Scholar 

  8. Sia IG, Berbari EF, Karchmer AW (2005) Prosthetic joint infections. Infect Dis Clin North Am 19:885–914

    Article  PubMed  Google Scholar 

  9. Zimmerli W, Trampuz A, Ochsner PE (2004) Prosthetic-joint infections. N Engl J Med 351:1645–1654

    Article  PubMed  CAS  Google Scholar 

  10. Anderl JN, Zahller J, Roe F et al (2003) Role of nutrient limitation and stationary-phase existence in Klebsiella pneumonia biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother 47:1251–1256

    Article  PubMed  CAS  Google Scholar 

  11. Ceri H, Olson ME, Stremick C et al (1999) The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J Clin Microbiol 37:1771–1776

    PubMed  CAS  Google Scholar 

  12. Montanaro L, Speziale P, Campoccia D et al (2011) Scenery of Staphylococcus implant infections in orthopedics. Future Microbiol 6:1329–1349

    Article  PubMed  CAS  Google Scholar 

  13. Dominguez-Herrera J, Docobo-Perez F, Lopez-Rojas R et al (2011) Efficacy of daptomycin versus vancomycin in an experimental model of foreign-body and systemic infection caused by biofilm producers and methicillin-resistant staphylococcus epidermidis. Antimicrob Agents Chemother 56:613–617

    Article  PubMed  Google Scholar 

  14. Del Pozo JL, Rouse MS, Euba G et al (2009) The electricidal effect is active in an experimental model of staphylococcus epidermidis chronic foreign body osteomyelitis. Antimicrob Agents Chemother 53:4064–4068

    Article  PubMed  Google Scholar 

  15. Ribeiro M, Monteiro F, Ferraz MP (2012) Infection of orthopedic implants with emphasis on bacterial adhesion process and techniques used in studying bacterial-material interactions. Biomatter 2:176–194

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the NIH National Institute of Allergy and Infectious Disease (NIAID) P01 AI083211 to Tammy Kielian.

Author information

Authors and Affiliations

  1. Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA

    Tyler D. Scherr, Carolyn R. Schaeffer, Mark L. Hanke & Tammy Kielian

  2. Department of Orthopedic Surgery, University of Nebraska Medical Center, Omaha, NE, USA

    Kevin E. Lindgren & Curtis W. Hartman

Authors
  1. Tyler D. Scherr
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  2. Kevin E. Lindgren
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  3. Carolyn R. Schaeffer
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  4. Mark L. Hanke
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  5. Curtis W. Hartman
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  6. Tammy Kielian
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Editor information

Editors and Affiliations

  1. Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, Nebraska, USA

    Paul D. Fey

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© 2014 Springer Science+Business Media, LLC

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Scherr, T.D., Lindgren, K.E., Schaeffer, C.R., Hanke, M.L., Hartman, C.W., Kielian, T. (2014). Mouse Model of Post-arthroplasty Staphylococcus epidermidis Joint Infection. In: Fey, P. (eds) Staphylococcus Epidermidis. Methods in Molecular Biology, vol 1106. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-736-5_16

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  • DOI: https://doi.org/10.1007/978-1-62703-736-5_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-735-8

  • Online ISBN: 978-1-62703-736-5

  • eBook Packages: Springer Protocols

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