Abstract
Chemical genomics is a highly effective approach for understanding complex and dynamic biological processes in plants. A chemical activity can be investigated by a reverse genetics strategy, for which a huge abundance and diversity of Arabidopsis thaliana mutants are readily available for exploitation. Here we present an approach to characterize a chemical of interest, as well as examples of studies demonstrating an effective combination of chemical genomics with reverse genetics strategies, drawn from recent literature on phytohormone signalling and auxin transport.
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We thank the Vetenskapsrådet and VINNOVA for supporting this work.
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Doyle, S.M., Robert, S. (2014). Using a Reverse Genetics Approach to Investigate Small-Molecule Activity. In: Hicks, G., Robert, S. (eds) Plant Chemical Genomics. Methods in Molecular Biology, vol 1056. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-592-7_6
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DOI: https://doi.org/10.1007/978-1-62703-592-7_6
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