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Use of Microarray Analysis to Investigate EMT Gene Signatures

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Adhesion Protein Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1046))

Abstract

The epithelial-to-mesenchymal transition (EMT) is a widely studied program of development of cells characterized by loss of cell adhesion, repression of E-cadherin expression, and increased cell mobility. Microarrays have become a well-established technique for simultaneously measuring the expression of thousands of transcripts encoded by the genome. In this chapter, we demonstrate how microarray analysis can be used to assess the role of EMT-genes associated with a collagen invading phenotype by generating a gene expression signature and relating this to cell line and tumor datasets from published microarray studies.

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Acknowledgments

This work was supported by Breakthrough Breast Cancer and the Scottish Funding Council.

Author information

Authors and Affiliations

  1. Breakthrough Breast Cancer Research Unit, Western General Hospital, Edinburgh, UK

    Andrew H. Sims & Alexey A. Larionov

  2. School of Medicine, University of St Andrews, St Andrews, UK

    David J. Harrison

  3. AMS Biotechnology, Abingdon, UK

    Elad Katz

Authors
  1. Andrew H. Sims
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  2. Alexey A. Larionov
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  3. David J. Harrison
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  4. Elad Katz
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Editor information

Editors and Affiliations

  1. , Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, OX3 7DQ, United Kingdom

    Amanda S. Coutts

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Sims, A.H., Larionov, A.A., Harrison, D.J., Katz, E. (2013). Use of Microarray Analysis to Investigate EMT Gene Signatures. In: Coutts, A. (eds) Adhesion Protein Protocols. Methods in Molecular Biology, vol 1046. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-538-5_5

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  • DOI: https://doi.org/10.1007/978-1-62703-538-5_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-537-8

  • Online ISBN: 978-1-62703-538-5

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