Abstract
Entosis is a recently described nonapoptotic cell death mechanism that is initiated by the engulfment of live epithelial cells, leading to the formation of “cell-in-cell” structures. Entotic cell engulfment is induced by matrix detachment, and is driven by imbalances in actomyosin contraction between neighboring cells. Here we describe methods to quantify the formation of cell-in-cell structures by entosis, for cells cultured in suspension or in soft agar, by fluorescence imaging and time-lapse microscopy.
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References
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Acknowledgment
This work was supported by a grant from the National Cancer Institute (CA154649; M.O.), The Geoffrey Beene Cancer Research Center at MSKCC (M.O.), the Louis V. Gerstner, and Jr. Young Investigators Fund (M.O.).
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Sun, Q., Overholtzer, M. (2013). Methods for the Study of Entosis. In: McCall, K., Klein, C. (eds) Necrosis. Methods in Molecular Biology, vol 1004. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-383-1_5
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DOI: https://doi.org/10.1007/978-1-62703-383-1_5
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Publisher Name: Humana Press, Totowa, NJ
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