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Evaluating Tumor Angiogenesis

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Pancreatic Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 980))

Abstract

The evaluation of tumor angiogenesis in pancreatic cancers involves determining the status of tumor vasculature and hypoxia in the tumor. Describing the nature and extent of tumor angiogenesis involves evaluating the expression of endothelial and perivascular cells within the tumor, and the expression of angiogenesis-related genes in tumor vasculature. Here we describe the methodology for assessment of tumor vasculature in murine mouse models of cancer. Specifically, we provide methodology for the evaluation of tumor hypoxia, tumor vessel perfusion, and chromogenic and fluorescent immunohistochemistry applied to tumor vascular analysis.

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Acknowledgments

This work was supported by NIH grant 5R01CA13667 (J.K.) and a Department of Defense Breast Cancer Program Predoctoral Fellowship DOD W81XWH-10-1-0304 (M.K.).

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Authors and Affiliations

  1. Department of Pharmacology, Columbia University Medical Center, New York, NY, USA

    Minji K. Uh

  2. Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY, USA

    Minji K. Uh & Jan Kitajewski

  3. Department of Surgery, Columbia University Medical Center, New York, NY, USA

    Jessica Kandel

  4. Division of Reproductive Sciences in Ob/Gyn, Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Jan Kitajewski

Authors
  1. Minji K. Uh
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  2. Jessica Kandel
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  3. Jan Kitajewski
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Corresponding author

Correspondence to Jan Kitajewski .

Editor information

Editors and Affiliations

  1. , Irving Cancer Research Center, Columbia University, St. Nicholas Avenue 1130, New York, 10032, New York, USA

    Gloria H. Su

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© 2013 Springer Science+Business Media, LLC

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Uh, M.K., Kandel, J., Kitajewski, J. (2013). Evaluating Tumor Angiogenesis. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_20

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  • DOI: https://doi.org/10.1007/978-1-62703-287-2_20

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-286-5

  • Online ISBN: 978-1-62703-287-2

  • eBook Packages: Springer Protocols

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