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Array-Based Yeast Two-Hybrid Screens: A Practical Guide

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Two Hybrid Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 812))

Abstract

Yeast two-hybrid screens are carried out as random library screens or matrix-based screens. The latter have the advantage of being better controlled and thus typically give clearer results. In this chapter, we provide detailed protocols for matrix-based Y2H screens and give some helpful instructions how to plan a large-scale interaction screen. We also discuss strategies to identify or avoid false negatives and false positives.

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Abbreviations

3-AT:

3-Amino-1,2,4-triazole

AD:

Activation domain

DBD:

DNA-binding domain

ORF:

Open reading frame

Y2H:

Yeast two hybrid

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Acknowledgments

Work on this paper was supported by NIH grant RO1GM79710, the Landesstiftung Baden-Württemberg (Germany), and the Seventh Research Framework Programme of the European Union (grant HEALTH-F3-2009-223101).

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Authors and Affiliations

  1. Karlsruhe Institute of Technology, Karlsruhe, Germany

    Roman Häuser & Thorsten Stellberger

  2. J Craig Venter Institute (JCVI), Rockville, MD, USA

    Seesandra V. Rajagopala

  3. Center for the Study of Biological Complexity Virginia Commonwealth University, Richmond, VA, USA

    Peter Uetz

Authors
  1. Roman Häuser
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  2. Thorsten Stellberger
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  3. Seesandra V. Rajagopala
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  4. Peter Uetz
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Corresponding author

Correspondence to Peter Uetz .

Editor information

Editors and Affiliations

  1. Molekulare Medizin (MDC), Max-Delbrück-Centrum für, Robert-Rössle-Str. 10, Berlin-Buch, 13125, Germany

    Bernhard Suter

  2. Max-Delbrück-Centrum-Für Molekulare Medi, Robert-Rössle-Strabe 10, Berlin, 13125, Berlin, Germany

    Erich E. Wanker

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Häuser, R., Stellberger, T., Rajagopala, S.V., Uetz, P. (2012). Array-Based Yeast Two-Hybrid Screens: A Practical Guide. In: Suter, B., Wanker, E. (eds) Two Hybrid Technologies. Methods in Molecular Biology, vol 812. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-455-1_2

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  • DOI: https://doi.org/10.1007/978-1-61779-455-1_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-454-4

  • Online ISBN: 978-1-61779-455-1

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