Abstract
Direct sequencing of RNA molecules using next-generation sequencing (NGS) technology has revolutionized the analysis of transcriptome with its massively parallel throughput and low cost. Here, we describe Illumina’s microRNA-Seq, a method for sequencing microRNA using the Illumina Genome Analyzer system. The sequence data generated from this method enables direct identifying and profiling of microRNAs in any given organism. It also sheds light in understanding the biogenesis and modification of microRNA.
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© 2012 Springer Science+Business Media, LLC
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Luo, S. (2012). MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform. In: Fan, JB. (eds) Next-Generation MicroRNA Expression Profiling Technology. Methods in Molecular Biology, vol 822. Humana Press. https://doi.org/10.1007/978-1-61779-427-8_12
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DOI: https://doi.org/10.1007/978-1-61779-427-8_12
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Publisher Name: Humana Press
Print ISBN: 978-1-61779-426-1
Online ISBN: 978-1-61779-427-8
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