Abstract
The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms. Basic cell-handling techniques are discussed, including choice of media, primary culture, and cryopreservation of cells so they can be stored for future use. Common assays which are used to determine cell viability and activity are considered.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Freshney, R.I., Culture of Animal Cells: A Manual of Basic Technique. Fourth ed. 2000, New York: Wiley-Liss, Inc.
Freshney, R.I., Introduction to basic principles. Second ed. Animal Cell Culture: A Practical Approach, ed. R.I. Freshney. 1994, Oxford: Oxford University Press. 1–14.
Eagle, H., Amino Acid Metabolism in Mammalian Cell Cultures. Science, 1959. 130: p. 432–437.
Dulbecco, R. and G. Freeman, Plaque production by the polyoma virus. Virology, 1959. 8: p. 396–397.
Moore, G.E., R.E. Gerner, and H.A. Franklin, Culture of Normal Human Leukocytes. Journal of the American Medical Association, 1967. 199: p. 519–524.
Freshney, R.I., Basic Principles of Cell Culture. Culture of Cells for Tissue Engineering, ed. G. Vunjak-Novakovic and R.I. Freshney. 2006, New York: John Wiley & Sons, Inc. 3–22.
Marcovic, O. and N. Marcovic, Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cellular & Develop-mental Biology, 1998. 34: p. 108.
Armitage, W.J., Cryopreservation of animal cells. Symposia of the Society for Experimental Biology, 1987. 41: p. 379–393.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Philippeos, C., Hughes, R.D., Dhawan, A., Mitry, R.R. (2012). Introduction to Cell Culture. In: Mitry, R., Hughes, R. (eds) Human Cell Culture Protocols. Methods in Molecular Biology, vol 806. Humana Press. https://doi.org/10.1007/978-1-61779-367-7_1
Download citation
DOI: https://doi.org/10.1007/978-1-61779-367-7_1
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-366-0
Online ISBN: 978-1-61779-367-7
eBook Packages: Springer Protocols