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Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method

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Peptide Microarrays

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 570))

Abstract

Peptide synthesis on cellulose using the SPOT technology follows the standard Fmoc-chemistry and can be performed manually or automated. This method allows the synthesis of low-cost peptide arrays containing around 900 large spots of addressable peptides on a cellulose sheet of 19 cm × 29 cm. These peptides can be cleaved from the cellulose support by ammonia gas and afterward spotted on glass microchips. Alternatively, the peptides can be synthesized on modified cellulose discs and CelluSpot microarrays can be produced.

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Author information

Authors and Affiliations

  1. Centre for Microbial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada

    Dirk F.H. Winkler, Kai Hilpert, Ole Brandt & Robert E.W. Hancock

Authors
  1. Dirk F.H. Winkler
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  2. Kai Hilpert
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  3. Ole Brandt
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  4. Robert E.W. Hancock
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Editor information

Editors and Affiliations

  1. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marina Cretich

  2. Inst. Chimica del Riconoscimento, CNR, Via Mario Bianco 9, Milano, 20131, Italy

    Marcella Chiari

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Winkler, D.F., Hilpert, K., Brandt, O., Hancock, R.E. (2009). Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method. In: Cretich, M., Chiari, M. (eds) Peptide Microarrays. Methods in Molecular Biology™, vol 570. Humana Press. https://doi.org/10.1007/978-1-60327-394-7_5

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  • DOI: https://doi.org/10.1007/978-1-60327-394-7_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-393-0

  • Online ISBN: 978-1-60327-394-7

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