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Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity

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Viral Applications of Green Fluorescent Protein

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 515))

Summary

Enhanced green fluorescent protein (EGFP) is a useful marker protein which enables the tracing of virus infection. Recombinant viruses expressing EGFP are useful for the investigation of the underlying mechanism of viral infection in vitro and in vivo. Using EGFP-expressing recombinant Nipah virus (NiV) and canine distemper virus (CDV), we tested the susceptibility of a variety of cells to infection. Receptor usage in CDV infection was also investigated.

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Acknowledgments

The authors gratefully acknowledge the assistance of P4 laboratory staffs for the treatment of live NiV in the INSERM Jean MĂ©rieux P4 laboratory. This study was supported by grants-in-aid from the Ministry of Education, Science, Culture and Sports of Japan.

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Authors and Affiliations

  1. Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Misako Yoneda, Kentaro Fujita, Hiroki Sato & Chieko Kai

Authors
  1. Misako Yoneda
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  2. Kentaro Fujita
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  3. Hiroki Sato
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  4. Chieko Kai
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Corresponding author

Correspondence to Chieko Kai .

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Editors and Affiliations

  1. Department of Chemistry, 2355 Fairchild Drive, US Airforce Academy, CO, 80840, USA

    Barry W. Hicks

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Yoneda, M., Fujita, K., Sato, H., Kai, C. (2009). Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity. In: Hicks, B.W. (eds) Viral Applications of Green Fluorescent Protein. Methods in Molecular Biology™, vol 515. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-559-6_23

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  • DOI: https://doi.org/10.1007/978-1-59745-559-6_23

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-87-9

  • Online ISBN: 978-1-59745-559-6

  • eBook Packages: Springer Protocols

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