Abstract
Toxoplasma gondii is an intracellular, apicomplexan parasite of great importance in both human and animal health. Current research has identified a variety of important and necessary factors specific to the parasite that enable it to infect and persist in a wide array of mammalian hosts. However, in order to continue to build our understanding of T. gondii pathogenesis, the relevance of these parasite characteristics needs continued investigation in animal models. In the following chapter, we present a model of intraperitoneal infection of C57BL/6 mice with T. gondii tachyzoites that, in C57BL/6 mice, elicits a strong acute immune response. Moreover, we present methods for sampling and analyzing peritoneal and bronchoalveolar lavage fluids in order to assess localized and systemic immune reactions to the parasite.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Meers S, Lagrou K, Theunissen K et al (2010) Myeloablative conditioning predisposes patients for toxoplasma gondii reactivation after allogeneic stem cell transplantation. Clin Infect Dis 50(8):1127–1134. https://doi.org/10.1086/651266
Aliberti J (2005) Host persistence: exploitation of anti-inflammatory pathways by toxoplasma gondii. Nat Rev Immunol 5(2):162–170. https://doi.org/10.1038/nri1547
Perez-Mazliah D, Langhorne J (2014) CD4 T-cell subsets in malaria: TH1/TH2 revisited. Front Immunol 5:671. https://doi.org/10.3389/fimmu.2014.00671
Leopold Wager CM, Hole CR, Wozniak KL et al (2014) STAT1 signaling is essential for protection against Cryptococcus neoformans infection in mice. J Immunol 193(8):4060–4071. https://doi.org/10.4049/jimmunol.1400318
Hong YH, Lillehoj HS, Lee SH et al (2006) Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections. Vet Immunol Immunopathol 114(3-4):209–223. https://doi.org/10.1016/j.vetimm.2006.07.007
Pfefferkorn ER, Kasper LH (1983) Toxoplasma gondii: genetic crosses reveal phenotypic suppression of hydroxyurea resistance by fluorodeoxyuridine resistance. Exp Parasitol 55(2):207–218
Hunter CA, Sibley LD (2012) Modulation of innate immunity by toxoplasma gondii virulence effectors. Nat Rev Microbiol 10(11):766–778. https://doi.org/10.1038/nrmicro2858
Scanga CA, Aliberti J, Jankovic D et al (2002) Cutting edge: MyD88 is required for resistance to toxoplasma gondii infection and regulates parasite-induced IL-12 production by dendritic cells. J Immunol 168(12):5997–6001
Mun HS, Aosai F, Norose K et al (2003) TLR2 as an essential molecule for protective immunity against toxoplasma gondii infection. Int Immunol 15(9):1081–1087
Gorfu G, Cirelli KM, Melo MB et al (2014) Dual role for inflammasome sensors NLRP1 and NLRP3 in murine resistance to toxoplasma gondii. mBio 5(1). https://doi.org/10.1128/mBio.01117-13
Coutermarsh-Ott SL, Doran JT, Campbell C et al (2016) Caspase-11 modulates inflammation and attenuates toxoplasma gondii pathogenesis. Mediat Inflamm 2016:9848263. https://doi.org/10.1155/2016/9848263
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Coutermarsh-Ott, S. (2019). Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_21
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9167-9_21
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9166-2
Online ISBN: 978-1-4939-9167-9
eBook Packages: Springer Protocols