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Establishment of Replication-Competent HCV Strain with Minimum Modifications

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Hepatitis C Virus Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1911))

Abstract

The HCV cell culture system, consisting of the JFH-1 strain and HuH-7 cells, has been broadly used to assess the complete HCV life cycle in cultured cells. However, being able to use multiple HCV strains in such a system is vital for future studies of this virus. We recently established a novel HCV cell culture system using another HCV genotype 2a strain, J6CF, which replicates in chimpanzees but not in cultured cells. We identified effective cell culture-adaptive mutations and established a replication-competent J6CF strain with minimum modifications in cultured cells. The strategy of how we established the replication-competent HCV strain and how we identified the effective cell culture-adaptive mutations is described here and could prove useful for establishing other replication-competent HCV strains.

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Authors and Affiliations

  1. Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan

    Takanobu Kato, Asako Murayama & Takaji Wakita

Authors
  1. Takanobu Kato
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  2. Asako Murayama
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  3. Takaji Wakita
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Corresponding author

Correspondence to Takaji Wakita .

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Editors and Affiliations

  1. Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA

    Mansun Law

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Kato, T., Murayama, A., Wakita, T. (2019). Establishment of Replication-Competent HCV Strain with Minimum Modifications. In: Law, M. (eds) Hepatitis C Virus Protocols . Methods in Molecular Biology, vol 1911. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8976-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8976-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8975-1

  • Online ISBN: 978-1-4939-8976-8

  • eBook Packages: Springer Protocols

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