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Methods for the Study of Entotic Cell Death

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Autophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1880))

Abstract

Entosis is a mechanism of cell competition occurring in cancers that involves the engulfment and killing of neighboring cells. The death of ingested cells, called entotic cell death, usually occurs in a non-apoptotic, autophagy protein-dependent manner, where microtubule-associated protein light chain 3 (LC3) is lipidated onto entotic vacuoles. Here we present methods to quantify entotic cell death and its associated LC3 lipidation.

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References

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Author information

Authors and Affiliations

  1. Cell Biology Program, Sloan Kettering Institute for Cancer Research, New York, NY, USA

    Jens C. Hamann, Sung Eun Kim & Michael Overholtzer

  2. Louis V. Gerstner, Jr. Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Jens C. Hamann

  3. Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA

    Sung Eun Kim

  4. Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA

    Jens C. Hamann

Authors
  1. Jens C. Hamann
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  2. Sung Eun Kim
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  3. Michael Overholtzer
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Corresponding author

Correspondence to Michael Overholtzer .

Editor information

Editors and Affiliations

  1. Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis

  2. Signalling Programme, Babraham Institute, Cambridge, UK

    Oliver Florey

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Hamann, J.C., Kim, S.E., Overholtzer, M. (2019). Methods for the Study of Entotic Cell Death. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_28

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  • DOI: https://doi.org/10.1007/978-1-4939-8873-0_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8872-3

  • Online ISBN: 978-1-4939-8873-0

  • eBook Packages: Springer Protocols

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