Abstract
Autophagy protects colorectal cancer cells against therapeutic intervention. Autophagy is a continuous process, and autophagic flux requires both autophagosome synthesis and their subsequent degradation at lysosomes. Hence, cells with elevated autophagic flux display both rapid autophagosome generation and degradation. Here, we describe an immunoblot protocol coupled to pharmaceutical inhibition of autophagosome clearance to monitor autophagic flux levels between colorectal cancer cell lines.
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Acknowledgments
This work was supported by grants from the Canadian Institutes of Health Research (CIHR) and the Cancer Research Society (CRS) to S.J. and by junior faculty salary awards from the CIHR and Fond de Recherche du Québec—Santé (FRQS) to S.J.. Steve Jean is a member of the FRSQ-Funded Centre de Recherche du CHUS.
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Lauzier, A., Jean, S. (2018). Autophagic Flux Assessment in Colorectal Cancer Cells. In: Beaulieu, JF. (eds) Colorectal Cancer. Methods in Molecular Biology, vol 1765. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7765-9_10
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DOI: https://doi.org/10.1007/978-1-4939-7765-9_10
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