Abstract
The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.
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References
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Mermaz, B., Liu, F., Song, J. (2018). RNA Immunoprecipitation Protocol to Identify Protein–RNA Interactions in Arabidopsis thaliana . In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_19
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DOI: https://doi.org/10.1007/978-1-4939-7318-7_19
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7318-7
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