Abstract
Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).
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Acknowledgments
This study was supported by grant from the National Science Center, Poland granted on the basis of decision number DEC-2012/07/N/NZ6/04118 and grant no. 612 427 from the Jan Kochanowski University. Some of the experiments were run on apparatus purchased with European Union grant 307 under 2.2 Innovation Industry.
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Żarnowiec, P., Czerwonka, G., Kaca, W. (2017). Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 1600. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6958-6_11
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DOI: https://doi.org/10.1007/978-1-4939-6958-6_11
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