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Array-Based Comparative Genomic Hybridization (aCGH)

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Cancer Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1541))

Abstract

Copy number variations (CNVs) in the genomes have been suggested to play important roles in human evolution, genetic diversity, and disease susceptibility. A number of assays have been developed for the detection of CNVs, including fluorescent in situ hybridization (FISH), array-based comparative genomic hybridization (aCGH), PCR-based assays, and next-generation sequencing (NGS). In this chapter, we describe a microarray method that has been used for the detection of genome-wide CNVs, loss of heterozygosity (LOH), and uniparental disomy (UPD) associated with constitutional and neoplastic disorders.

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Acknowledgment

We would like to thank Dr. Charles Lee and other colleagues for their kind help and support.

Author information

Authors and Affiliations

  1. The Jackson Laboratory for Genomic Medicine, 10 Discovery Drive, Farmington, CT, 06032, USA

    Chengsheng Zhang M.D., Ph.D., Eliza Cerveira, Mallory Romanovitch & Qihui Zhu

Authors
  1. Chengsheng Zhang M.D., Ph.D.
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  2. Eliza Cerveira
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  3. Mallory Romanovitch
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  4. Qihui Zhu
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Corresponding author

Correspondence to Chengsheng Zhang M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. Haematology Division Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong Prince of Wales Hospital, Shatin, Hong Kong, China

    Thomas S.K. Wan

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Zhang, C., Cerveira, E., Romanovitch, M., Zhu, Q. (2017). Array-Based Comparative Genomic Hybridization (aCGH). In: Wan, T. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 1541. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6703-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6703-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6701-8

  • Online ISBN: 978-1-4939-6703-2

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