Abstract
Metabolic flexibility is vital for the cells to adapt to different energetic situations, allowing the organisms to adapt to changing conditions and survive challenges. One of the most important regulators of the metabolic flexibility is PGC-1α activity. PGC-1α integrates numerous signals and regulates a variety of transcription factors and nuclear receptors that together regulate mitochondrial homeostasis and fatty acid oxidation. One of the major ways that PGC-1α activity is regulated is by changes in its acetylation status. Thus measuring the acetylation status of PGC-1α is an important indicator of the metabolic flexibility of the cells. In this chapter, we describe an approach to evaluate PGC-1α acetylation in primary mouse myotubes. The method is applicable to other cell types and tissues as well.
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Acknowledgements
We would like to thank Eric L. Bell for helpful advice on the immunoprecipitation and acetylation detection protocol. This work was supported by a fellowship from the Portuguese Foundation for Science and Technology (SFRH/BD/44674/2008) to A.P.G, the Paul F. Glenn Foundation for Medical Research, the United Mitochondrial Disease Foundation, The Juvenile Diabetes Research Foundation, and NIA/NIH grants to D.A.S.
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Gomes, A.P., Sinclair, D.A. (2015). Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes. In: Palmeira, C., Rolo, A. (eds) Mitochondrial Regulation. Methods in Molecular Biology, vol 1241. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1875-1_5
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DOI: https://doi.org/10.1007/978-1-4939-1875-1_5
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