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DESI Imaging of Small Molecules in Biological Tissues

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Mass Spectrometry Imaging of Small Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1203))

Abstract

Desorption electrospray ionization (DESI) allows the direct analysis of ordinary objects or preprocessed samples under ambient conditions. Among other applications, DESI is used to identify and to record spatial distributions of small molecules in situ, sliced or imprinted biological tissue. Manipulation of the chemistry accompanying ambient analysis ionization can be used to optimize chemical analysis, including molecular imprinting. Images are obtained by continuously moving the sample relative to the DESI sprayer and the inlet of the mass spectrometer. The acquisition time depends on the size of the surface to be analyzed and on the desired resolution.

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Acknowledgments

We thank the Brazilian National Council for Scientific and Technological Development (CNPq) and Natural Science and Engineering Research Council of Canada (NSERC).

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Centre for Research in Mass Spectrometry (CRMS), York University, 256 Chemistry Building, 4700 Keele Street, Toronto, ON, Canada, M3J 1P3

    Elaine C. Cabral & Demian R. Ifa

Authors
  1. Elaine C. Cabral
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  2. Demian R. Ifa
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Corresponding author

Correspondence to Demian R. Ifa .

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Editors and Affiliations

  1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA

    Lin He

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Cabral, E.C., Ifa, D.R. (2015). DESI Imaging of Small Molecules in Biological Tissues. In: He, L. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 1203. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1357-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1357-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1356-5

  • Online ISBN: 978-1-4939-1357-2

  • eBook Packages: Springer Protocols

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