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Transcriptional Regulation in Adipogenesis Through PPARγ-Dependent and -Independent Mechanisms by Prostaglandins

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Transcription Factor Regulatory Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1164))

Abstract

Adipogenesis is controlled by complex mechanisms, and transcription factors are involved in its regulation. PPARγ is a ligand-dependent transcription factor and the most important one for adipogenesis. Although prostaglandin (PG) D2 metabolites have been reported as being the ligands of PPARγ, the endogenous PPARγ ligand in adipocytes remains unclear. Here, we show the methods for the general analysis of adipocyte differentiation and the protocols for promoter analysis, fluorescence EMSA, and chromatin immunoprecipitation assay for the transcriptional regulation of the SREBP-1c-activated lipocalin-type PGD synthase gene in adipocytes. Moreover, we describe that PGD2 and its metabolites are involved in the regulation of adipogenesis through PPARγ-dependent and -independent mechanisms.

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Acknowledgements

This work is supported in part by grants from the programs Genome Network Project and Grant in Aid for Scientific Research and Scientific Research on Innovative Areas of the Ministry of Education, Culture, Sports, Science and Technology of Japan and Technology of Japan (MEXT).

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Authors and Affiliations

  1. Laboratory of Biodefense and Regulation, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka, 569-1094, Japan

    Ko Fujimori

  2. Department of Molecular Behavioral Biology, Osaka Bioscience Institute, 6-2-4 Furuedai, Suita, Osaka, 565-0874, Japan

    Ko Fujimori & Yoshihiro Urade

  3. Department of Molecular Sleep Biology, International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

    Yoshihiro Urade

Authors
  1. Ko Fujimori
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  2. Yoshihiro Urade
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Corresponding author

Correspondence to Yoshihiro Urade .

Editor information

Editors and Affiliations

  1. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Etsuko Miyamoto-Sato

  2. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hiroyuki Ohashi

  3. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Hirotaka Sasaki

  4. Division of Interactome Medical Science, The University of Tokyo, The Institute of Medical Science, Minato-ku, Tokyo, Japan

    Jun-ichi Nishikawa

  5. Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan

    Hiroshi Yanagawa

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Fujimori, K., Urade, Y. (2014). Transcriptional Regulation in Adipogenesis Through PPARγ-Dependent and -Independent Mechanisms by Prostaglandins. In: Miyamoto-Sato, E., Ohashi, H., Sasaki, H., Nishikawa, Ji., Yanagawa, H. (eds) Transcription Factor Regulatory Networks. Methods in Molecular Biology, vol 1164. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0805-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-0805-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0804-2

  • Online ISBN: 978-1-4939-0805-9

  • eBook Packages: Springer Protocols

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