Abstract
The preparation of hydrogel films with immobilized yeast cells and their use as membrane in a reactor is discussed. The permeate flow rate, which is initially high, decreases with operational time and reaches an asymptotic value which linearly depends on the applied pressure in the investigated range (0.1–4 atm). The rate of flux decay was shown to be a linear inverse function of time and of a decay constant K.
The yeast invertase activity present in the membrane was monitored and the experimental values of the Michaelis constant, km = 60 mM, and the activation energy, Ea = 7367 cal/mol, show that enzyme kinetics is close to that observed with free cells.
The operational thermostability was measured in the membrane reactor at 45°C and the invertase activity is significantly high for a prolonged time.
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References
F. Alfani, M. Cantarella, and V. Scardi, Use of a membrane reactor for studying enzymatic hydrolysis of cellulose, J.Membr.Sci., 16:407 (1983).
M. Cantarella, A. Gallifuoco, V. Scardi, and F. Alfani, Enzyme stability and glucose inhibition in cellulose saccharification, in Ann. N.Y. Acad. Sci., J. Hitchcock, ed., in press (1984).
M. Cantarella, C. Migliaresi, M. G. Tafuri, and F. Alfani, Immobilization of yeast cells in hydroxyethylmethacrylate gels, Applied Microb. and Biotechnol., in press (1984).
N. Nelson, A photometric adoption of the Samogyi method for the determination of glucose, J.Biol.Chem., 153:375 (1944).
V. Scardi, M. Cantarella, L. Gianfreda, R. Palescandolo, F. Alfani, and G. Greco, Enzyme immobilization by means of ultrafiltration techniques, Biochimie, 62:635 (1980).
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© 1986 Springer Science+Business Media New York
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Cantarella, M., Scardi, V., Gallifuoco, A., Tafuri, M.G., Alfani, F. (1986). Yeast Cell Entrapment in Polymeric Hydrogels: a Kinetic Study in Membrane Reactors. In: Drioli, E., Nakagaki, M. (eds) Membranes and Membrane Processes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-2019-5_24
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DOI: https://doi.org/10.1007/978-1-4899-2019-5_24
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