Abstract
Mutagenesis through the insertion of transposons has proved to be an invaluable technique for mapping the genes of complex plasmids1. No selection for a mutant phenotype has to be devised, but a straightforward selection for the antibiotic resistance markers, encoded by the transposon, is sufficient to identify the presence of a mutant plasmid.
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Leemans, J. et al. (1981). Plasmid Mobilization as a Tool for in Vivo Genetic Engineering. In: Levy, S.B., Clowes, R.C., Koenig, E.L. (eds) Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3983-0_38
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DOI: https://doi.org/10.1007/978-1-4684-3983-0_38
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