Abstract
We have developed a simple and rapid method for the preparation of post-synaptic densities (PSD’s) and synaptic junctional complexes from mammalian brain. By morphological criteria the PSD preparation is pure, being essentially free of organised membrane and other contaminants and the yield is high (70 μg protein/g wet weight starting material). The method involves the use of the relatively mild neutral detergent, n-octyl-glucoside (OG) in conjunction with phase partitioning.
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References
Cotman, C.W., and Taylor, D., 1972, Isolation and structural studies on synaptic complexes from rat brain, J. Cell Biol. 55: 696.
Gurd, J.W., 1981, Subcellular distribution and partial characterization of the three major classes of Concanavalin A receptors associated with rat brain synaptic junctions, Can. J. Biochem. 58: 941.
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© 1982 Springer Science+Business Media New York
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Gurd, J.W., Gordon-Weeks, P.R., Evans, W.H. (1982). Preparation of Synaptic Junctional Structures by Phase Partitioning in the Presence of n-Octyl-Glucoside. In: Bradford, H.F. (eds) Neurotransmitter Interaction and Compartmentation. NATO Advanced Study Institutes Series, vol 48. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-1140-9_2
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DOI: https://doi.org/10.1007/978-1-4684-1140-9_2
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-1142-3
Online ISBN: 978-1-4684-1140-9
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