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Post-translationally modified 14-3-3 isoforms and inhibition of protein kinase C

  • Chapter
Signal Transduction Mechanisms

Part of the book series: Developments in Molecular and Cellular Biochemistry ((DMCB,volume 15))

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Abstract

This report compares the ability of individual members of the 14-3-3 protein family to inhibit particular protein kinase C (PKC) isoforms. We also show that two of these 14-3-3 isoforms (α and δ) specific to mammalian and avian brain are in vivo post- translationally modified forms of β and ζ, respectively. The presence of this modification enhances the activity of 14-3-3 as an inhibitor of protein kinase C nearly two fold.

A method for analysing isoforms of 14-3-3 on acid-urea gels is also described. This permits the complete separation of all major isoforms and their unequivocal identification by a range of isoform specific antisera. The activity of recombinant 14-3- 3 and isoforms renatured by a novel method after separation by reverse phase HPLC are compared. The effects of diacylglycerol and the phorbol ester, PMA (phorbol 1 2-myristate 13 acetate) on the inhibition suggest that one of the sites of interaction of 14-3-3 may be the cysteine-rich (Cl) domain in PKC.

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Author information

Author notes

  1. Joel Madrazo

    Present address: CIGB, Ave.31, Po. Box 6162, Cubanacan, Habana, Cuba

Authors and Affiliations

  1. Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    Alastair Aitken, Steve Howell, David Jones, Joel Madrazo, Harry Martin, Yasmina Patel & Karen Robinson

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  1. Alastair Aitken
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  2. Steve Howell
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  3. David Jones
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  4. Joel Madrazo
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  5. Harry Martin
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  6. Yasmina Patel
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  7. Karen Robinson
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, University of West Indies, St. Augustine, Trinidad, West Indies, Caribbean

    Junor A. Barnes  & Haldane G. Coore  & 

  2. Department of Geriatric Medicine, Karolinska Institute, Stockholm, Sweden

    Abdul H. Mohammed

  3. Department of Pathology College of Medicine Saskatoon Cancer Center, University of Saskatoon, Saskatoon, Saskatchewan, Canada

    Rajendra K. Sharma

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© 1995 Springer Science+Business Media Dordrecht

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Aitken, A. et al. (1995). Post-translationally modified 14-3-3 isoforms and inhibition of protein kinase C. In: Barnes, J.A., Coore, H.G., Mohammed, A.H., Sharma, R.K. (eds) Signal Transduction Mechanisms. Developments in Molecular and Cellular Biochemistry, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2015-3_5

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  • DOI: https://doi.org/10.1007/978-1-4615-2015-3_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-5833-6

  • Online ISBN: 978-1-4615-2015-3

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