Abstract
We have previously shown that chronic supplementation of taurine to mice significantly ameliorated the age-dependent decline in memory acquisition and retention. We also showed that concomitant with the amelioration in cognitive function, taurine caused significant alterations in the GABAergic and somatonergic system. These changes include increased levels of the neurotransmitters GABA and glutamate, increased expression of both isoforms of GAD and the neuropeptide somatostatin, decreased hippocampal expression of the beta (β) 2/3 subunits of the GABAA receptor, an increase in the number of somatostatin-positive neurons, and an increase in the amplitude and duration of population spikes recorded from CA1 in response to Schaefer collateral stimulation and enhanced paired pulse facilitation in the hippocampus. These specific alterations of the inhibitory system caused by taurine treatment oppose those naturally induced by aging, suggesting a protective role of taurine in this process. In this study, we further investigated the effects of taurine on gene expression of relevant proteins of the inhibitory synapses using qRT-PCR method and found that taurine affects gene expression of various subunits of the GABAA receptors and GAD. Increased understanding the effects of taurine on gene expression will increase our understanding of age-related taurine-mediated neurochemical changes in the GABAergic system and will be important in elucidating the underpinnings of the functional changes of aging. Taurine might help forestall the age-related decline in cognitive functions through interaction with the GABAergic system.
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Abbreviations
- qRT-PC:
-
Quantitative reverse transcriptase polymerase chain reaction
- GAD:
-
Glutamic acid decarboxylase
- GABA:
-
γ-Aminobutyric acid
References
Andrew H, Zhang A, Ke Y, El Idrissi A, Shen C-H (2012) Decreased expression of GABAAβ Subunits in the brains of mice lacking the fragile X mental retardation protein. J Mol Neurosci 46:272–275
El Idrissi A, Trenkner E (2004) Taurine as a modulator of excitatory and inhibitory neurotransmission. Neurochem Res 29:189–197
Erlander MG, Tillakaratne NJK, Feldblum S, Patel N, Tobin AJ (1991) Two genes encode distinct glutamate decarboxylases. Neuron 7:91–100
Ford J, Odeyale O, Eskandar A, Kouba N, Shen C-H (2007) A SWI/SNF- and INO80-dependent nucleosome movement at the INO1promoter. Biochem Biophys Res Commun 361:974–979
Gleich O, Hamann I, Klump GM, Kittel M, Strutz J (2003) Boosting GABA improves impaired auditory temporal resolution in the gerbil. Neuroreport 14:1877–1880
Huxtable RJ (1992) Physiological actions of taurine. Physiol Rev 72:101–163
Leventhal AG, Wang Y, Pu M, Zhou Y, Ma Y (2003) GABA and its agonists improved visual cortical function in senescent monkeys. Science 300:812–815
Nishimura T, Schwarzer C, Furtinger S, Imai H, Kato N, Sperk G (2001) Changes in the GABA-ergic system induced by trimethyltin application in the rat. Mol Brain Res 97:1–6
Ramirez M, Gutierrez R (2001) Activity-dependent expression of GAD67 in the granule cells of the rat hippocampus. Brain Res 917:139–146
Riback CE, Byun MY, Ruiz GT, Reiffenstein RJ (1988) Increased levels of amino acid neurotransmitters in the inferior colliculus of the genetically epilepsy-prone rat. Epilepsy Res 2:9–13
Riback CE, Lauterborn JC, Navetta MS, Gall CM (1993) The inferior colliculus of GEPRs contains greater numbers of cells that express glutamate decarboxylase (GAD67) mRNA. Epilepsy Res 14:105–113
Satoh H, Sperelakis N (1998) Review of some actions of taurine on ion channels of cardiac muscle cells and others. Gen Pharmacol 30:451–463
Schaffer S, Takahashi K, Azuma J (2000) Role of osmoregulation in the actions of taurine. Amino Acids 19:527–546
Wimalarathna RN, Tsai C-H, Shen C-H (2011) Transcriptional regulation of genes involved in yeast phospholipid biosynthesis. J Microbiol 49:265–273
Zhang A, Shen C-H, Ma S-Y, Ke Y, El Idrissi A (2009) Altered expression of Autism-associated genes in the brain of Fragile X mouse model. Biochem Biophys Res Commun 379:920–923
Acknowledgments
This work was supported by an NSF Grant (MCB-0919218) and a PSC-CUNY award (64243-0042) to C. H. S. the College of Staten Island/CUNY and CDN-IBR.
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Shen, C.H., Lempert, E., Butt, I., Neuwirth, L.S., Yan, X., Idrissi, A.E. (2013). Changes in Gene Expression at Inhibitory Synapses in Response to Taurine Treatment. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 775. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6130-2_16
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DOI: https://doi.org/10.1007/978-1-4614-6130-2_16
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