Abstract
A major difficulty in studies of the fate of non-indigenous bacteria released into the environment is related to detection. Two common detection methods are selective plating on agar media (SP), and quantitative immunofluorescence microscopy (IF). Both methods have drawbacks; SP techniques rely on the ability of the bacteria to grow on laboratory media, whereas difficulties in separating cells from soil particles may limit the applicability of IF techniques. Furthermore IF does not distinguish between living and dead cells. Comparisons of SP and IF bacterial counts have shown varying results.
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© 1992 Plenum Press, New York
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Pedersen, J.C., Leser, T.D. (1992). Survival of Enterobacter Cloacae on Leaves and in Soil Detected by Immunofluorescence Microscopy in Comparison with Selective Plating. In: Stewart-Tull, D.E.S., Sussman, M. (eds) The Release of Genetically Modified Microorganisms—REGEM 2. Federation of European Microbiological Societies Symposium Series, vol 63. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-0493-7_49
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DOI: https://doi.org/10.1007/978-1-4613-0493-7_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-7654-8
Online ISBN: 978-1-4613-0493-7
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