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Recombinant Anti-idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development

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Proteomic Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2261))

Abstract

Sensitive and reproducible pharmacokinetic (PK) assays and immunogenicity assessment are required as part of the complex and lengthy development process for biotherapeutic proteins. Ligand binding assays (LBAs) are included in a range of approaches applied to understand the nature and properties of the drug as well as the induction of anti-drug antibodies (ADA) against the therapeutic, which can cause adverse events and loss of efficacy. Currently, most biotherapeutics are monoclonal human or humanized antibodies. Anti-idiotypic antibodies, targeting the idiotopic determinants of individual antibody drugs are recognized as perfect reagents for such LBAs. Here we describe the typical setups for these assays and how different types of anti-biotherapeutic antibodies can be used to establish selective and sensitive assays.

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Acknowledgments

We thank Amanda Turner and Achim Knappik for carefully reading the manuscript. All images are reprinted with permission of Bio-Rad Laboratories Inc., Hercules, CA, USA.

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Authors and Affiliations

  1. Bio-Rad AbD Serotec GmbH, Puchheim, Germany

    Stefan Harth & Christian Frisch

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  1. Stefan Harth
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  2. Christian Frisch
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Correspondence to Christian Frisch .

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Editors and Affiliations

  1. Bio-Rad Laboratories GmbH, Feldkirchen, Germany

    Anton Posch

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Harth, S., Frisch, C. (2021). Recombinant Anti-idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 2261. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1186-9_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1186-9_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1185-2

  • Online ISBN: 978-1-0716-1186-9

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