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Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining

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Germline Development in the Zebrafish

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2218))

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Abstract

Immunohistochemistry has been widely used as a robust technique to determine the cellular and subcellular localization of proteins. This information ultimately helps to understand the function of these proteins and how biological processes are regulated. Antibodies applicable for labeling in zebrafish are limited, making immuno-staining challenging. Recently glyoxal fixation was rediscovered in tissue culture, mouse, rat, and Drosophila, expanding the list of effective antibodies for these species. Here, we compare a protocol for zebrafish staining using glyoxal as a fixative agent with PFA. We demonstrate that glyoxal fixation improves the antigenicity of some epitopes thereby increasing the number of useful antibodies in zebrafish.

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Authors and Affiliations

  1. Institute of Human Genetics, University of Göttingen Medical Center, Georg-August-Universität, Göttingen, Germany

    Nadia Rostam

  2. Institute for Developmental Biochemistry, University of Göttingen Medical Center, Georg-August-Universität, Göttingen, Germany

    Roland Dosch

  3. Department of Developmental Biology, Johann-Friedrich-Blumenbach Institute of Zoology and Anthropology, Göttingen Center of Molecular Biosciences, Göttingen, Germany

    Nadia Rostam

  4. Department of Biology, College of Science, University of Sulaimani (UoS), Sulaimaniyah, Iraq

    Nadia Rostam

Authors
  1. Nadia Rostam
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  2. Roland Dosch
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Corresponding authors

Correspondence to Nadia Rostam or Roland Dosch .

Editor information

Editors and Affiliations

  1. Institute for Developmental Biochemistry, University of Göttingen Medical Center Georg-August-Universität, Göttingen, Germany

    Roland Dosch

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Rostam, N., Dosch, R. (2021). Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining . In: Dosch, R. (eds) Germline Development in the Zebrafish. Methods in Molecular Biology, vol 2218. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0970-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0970-5_19

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0969-9

  • Online ISBN: 978-1-0716-0970-5

  • eBook Packages: Springer Protocols

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