Abstract
In monoclonal antibody (mAb) production, aggregates represent a major class of product-related impurities that needs to be removed by the downstream process. Protein A chromatography is generally less effective at removing antibody aggregates under typical conditions, and in most cases aggregate removal relies on a subsequent polishing chromatography. Here we describe a procedure for effective removal of antibody aggregates using the mixed-mode chromatography resin Capto MMC ImpRes. Clearance of aggregates was confirmed by analytical size-exclusion chromatography (SEC) and native gel electrophoresis.
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Acknowledgments
We would like to thank Weichang Zhou and Peter (Keqiang) Shen for lending their support to this work.
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Chen, T., Guo, G., Tan, G., Wang, Y., Li, Y. (2021). Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin. In: Labrou, N.E. (eds) Protein Downstream Processing. Methods in Molecular Biology, vol 2178. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0775-6_23
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DOI: https://doi.org/10.1007/978-1-0716-0775-6_23
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Publisher Name: Humana, New York, NY
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