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Semi-In Vivo Assay for Pollen Tube Attraction

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Pollen and Pollen Tube Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2160))

Abstract

In flowering plants, each pollen tube delivers two sperm cells into the ovule to complete double fertilization. During the process, pollen tubes need to be navigated into the ovule, where accurate and complex pre-ovule guidance and ovule guidance are required. In recent years, different methods have been established to study those genes involved in the regulation of pollen tube guidance. Semi-in vivo ovule targeting mimics in vivo pollen tube micropylar guidance, and the semi-in vivo ovule targeting assay has been used to investigate function of genes involved in micropylar guidance. Moreover, the ovule targeting assay is the best way to do live cell imaging, which facilitates observation of pollen tube reception, synergid cell degeneration, and semi-in vivo gamete fusion. Meanwhile, semi-in vivo pollen tube attraction assay is another useful method to directly determine whether a certain molecule has pollen tube attraction activity.

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Acknowledgements

The research in the Qu laboratory is supported by grants from National Natural Science Foundation of China (31830004, 31620103903 and 31621001) and by the Peking-Tsinghua Joint Center for Life Sciences.

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Authors and Affiliations

  1. State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences at College of Life Sciences, Peking University, Beijing, People’s Republic of China

    Sheng Zhong, Zhijuan Wang & Li-Jia Qu

  2. National Plant Gene Research Center, Peking University, Beijing, People’s Republic of China

    Li-Jia Qu

Authors
  1. Sheng Zhong
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  2. Zhijuan Wang
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  3. Li-Jia Qu
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Corresponding author

Correspondence to Li-Jia Qu .

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Editors and Affiliations

  1. Department of Plant Science, McGill University, Montreal, QC, Canada

    Anja Geitmann

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Zhong, S., Wang, Z., Qu, LJ. (2020). Semi-In Vivo Assay for Pollen Tube Attraction. In: Geitmann, A. (eds) Pollen and Pollen Tube Biology. Methods in Molecular Biology, vol 2160. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0672-8_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0672-8_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0671-1

  • Online ISBN: 978-1-0716-0672-8

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