Abstract
Bioprinting is an additive manufacturing process where biomaterials-based inks are printed layer-by-layer to create three-dimensional (3D) structures that mimic natural tissues. Quality assurance for 3D bioprinting is paramount to undertaking fundamental research and preclinical and clinical product development. It forms part of quality management and is vital to reproducible and safe tissue fabrication, function, and regulatory approval for translational application. This chapter seeks to place the implementation of quality practices in 3D bioprinting front-of-mind, with emphasis on cell processing, although important to all components and procedures of the printing pipeline.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gu Q, Tomaskovic-Crook E, Lozano R, Chen Y, Kapsa RM, Zhou Q et al (2016) Functional 3D neural mini-tissues from printed gel-based human neural stem cells. Adv Healthc Mater 5:1429–1438
Gu Q, Tomaskovic-Crook E, Wallace GG, Crook JM (2017) 3D bioprinting human induced pluripotent stem cell constructs for in situ cell proliferation and successive multi-lineage differentiation. Adv Healthc Mater 6:1700175
Tomaskovic-Crook E, Zhang P, Ahtiainen A, Kaisvuo H, Lee CY, Beirne S et al (2019) Human neural tissues from neural stem cells using conductive biogel and printed polymer microelectrode arrays for 3D electrical stimulation. Adv Healthc Mater 8(15):1900425
Tomaskovic-Crook E, Crook JM (2018) Clinically-amendable defined and rapid induction of human brain organoids from induced pluripotent stem cells. In: Turksen K (ed) Organoids, Methods in Molecular Biology Series, 1st edn. Springer Nature, New York, p 1576
Crook JM, Peura TT, Kravets L, Bosman AG, Buzzard JJ, Horne R et al (2007) The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell 1(5):490–494
Acknowledgments
The author wishes to acknowledge funding from the Australian Research Council (ARC) Centre of Excellence Scheme (CE140100012).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Crook, J.M. (2020). Cell Processing for 3D Bioprinting: Quality Requirements for Quality Assurance in Fundamental Research and Translation. In: Crook, J.M. (eds) 3D Bioprinting. Methods in Molecular Biology, vol 2140. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0520-2_2
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0520-2_2
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0519-6
Online ISBN: 978-1-0716-0520-2
eBook Packages: Springer Protocols