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Lipidomics by HILIC-Ion Mobility-Mass Spectrometry

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Ion Mobility-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2084))

Abstract

Lipidomics is a rapidly growing field that enables the characterization of the entire lipidome in cells, tissues, or an organism. Changes in lipid metabolism and homeostasis caused by different disease states or drug treatments can be probed by lipidomics experiments, which can aid our understanding of normal physiology and disease pathology at the molecular level. While current technologies using liquid chromatography coupled with high-resolution mass spectrometry have greatly increased coverage of the lipidome, there are still limitations in resolving the large number of lipid species with similar masses in a narrow mass window. We recently reported that two orthogonal separation techniques, hydrophilic interaction liquid chromatography (HILIC) and ion mobility (IM), enhance the resolution of lipid species based on headgroup polarity and gas-phase size and shape, respectively, of various classes of glycerolipids, glycolipids, phospholipids, and sphingolipids. Here we describe the application of our HILIC-IM-MS lipidomics protocol to the analysis of lipid extracts derived from either tissues or cells, to identify significant changes in the lipidome in response to an internal or external stimulus, such as exposure to environmental chemicals.

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Acknowledgements

This work was supported by grants from National Institutes of Health (R00HD073270 and R01HD092659). A.L. is an appointed trainee of the Pharmacological Sciences Training Program funded by the National Institutes of Health (T32GM007750).

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Authors and Affiliations

  1. Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA

    Amy Li, Kelly M. Hines & Libin Xu

Authors
  1. Amy Li
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  2. Kelly M. Hines
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  3. Libin Xu
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Corresponding author

Correspondence to Libin Xu .

Editor information

Editors and Affiliations

  1. Department of Medicine and Surgery, University of Milano-Bicocca, Vedano al Lambro, Monza e Brianza, Italy

    Giuseppe Paglia

  2. Georgetown University, Washington, DC, USA

    Giuseppe Astarita

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Li, A., Hines, K.M., Xu, L. (2020). Lipidomics by HILIC-Ion Mobility-Mass Spectrometry. In: Paglia, G., Astarita, G. (eds) Ion Mobility-Mass Spectrometry . Methods in Molecular Biology, vol 2084. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0030-6_7

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  • DOI: https://doi.org/10.1007/978-1-0716-0030-6_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0029-0

  • Online ISBN: 978-1-0716-0030-6

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