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Technologies for the Global Discovery and Analysis of Alternative Splicing

  • Chapter
Alternative Splicing in the Postgenomic Era

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 623))

Abstract

During the past ∼20 years, studies on alternative splicing (AS) have largely been directed at the identification and characterization of factors and mechanisms responsible for the control of splice site selection, using model substrates and on a case by case basis. These studies have provided a wealth of information on the factors and interactions that control formation of the spliceosome. However, relatively little is known about the global regulatory properties of AS. Important questions that need to be addressed are: which exons are alternatively spliced and under which cellular contexts, what are the functional roles of AS events in different cellular contexts, and how are AS events controlled and coordinated with each other and with other levels of gene regulation to achieve cell- and developmentspecific functions. During the past several years, new technologies and experimental strategies have provided insight into these questions. For example, custom microarrays and data analysis took are playing a prominent role in the discovery and analysis of splicing regulation. Moreover, several non-microarray-based technologies are emerging that will likely further fuel progress in this area. This review focuses on recent advances made in the development and application of high-throughput methods to study AS.

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Authors and Affiliations

  1. Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics. Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Canada, MSS 3E1

    John A. Calarco, Arneet L. Saltzman, Joanna Y. Ip & Benjamin J. Blencowe

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  1. John A. Calarco
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  2. Arneet L. Saltzman
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  3. Joanna Y. Ip
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  4. Benjamin J. Blencowe
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Editors and Affiliations

  1. Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, Terrence Donnelly Center for Cellular and Biomedical Research, University of Toronto, Toronto, Canada

    Benjamin J. Blencowe Ph.D.

  2. Department of Genetics and Development Biology, University of Connecticut Health Center, Farmington, Connecticut, USA

    Brenton R. Graveley Ph.D.

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Calarco, J.A., Saltzman, A.L., Ip, J.Y., Blencowe, B.J. (2007). Technologies for the Global Discovery and Analysis of Alternative Splicing. In: Blencowe, B.J., Graveley, B.R. (eds) Alternative Splicing in the Postgenomic Era. Advances in Experimental Medicine and Biology, vol 623. Springer, New York, NY. https://doi.org/10.1007/978-0-387-77374-2_5

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  • DOI: https://doi.org/10.1007/978-0-387-77374-2_5

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  • Print ISBN: 978-0-387-77373-5

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