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The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis

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Abstract

Acute pancreatitis (AP) is an acute inflammatory condition that results from the digestion of pancreatic tissue by its own enzymes released from the acinar cells. The objective of this study was to investigate the effects of resveratrol on oxidative damage, pro-inflammatory cytokines, and tissue injury involved with AP induced in a rat model using sodium taurocholate (n = 60). There were three treatment groups with 20 rats per group. Groups I and II received 3 % sodium taurocholate solution, while group III underwent the same surgical procedure yet did not receive sodium taurocholate. In addition, group II received 30 mg/kg resveratrol solution. Rats were sacrificed at 2, 6, 12, and 24 h time points following the induction of AP. Blood and pancreatic tissue samples were collected and subjected to biochemical assays, Western blot assays, and histopathologic evaluations. Resveratrol did not reduce trypsin levels and prevent tissue damage. Resveratrol prevented IκB degradation (except for 6 h) and decreased nuclear factor-κB (NF-κB), activator protein-1 (AP-1) (except for 24 h), and levels of TNF-α, IL-6 (except for 24 h), and iNOS in the pancreatic tissue at all time points (P < 0.05). Serum nitric oxide (NO) levels were reduced as well (P < 0.05). Thus, we concluded that resveratrol did not reduce trypsin levels and did not prevent tissue injury despite the reduction in oxidative damage and pro-inflammatory cytokine levels detected in this model of AP.

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Acknowledgement

This study was supported by the Research Fund of Istanbul University (project number 2700/30042008).

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Authors and Affiliations

  1. Department of Pathology, Faculty of Veterinary Medicine, Istanbul University, 34320, Avcilar,, Istanbul, Turkey

    Ahmet Gulcubuk, Damla Haktanir & Funda Yildirim

  2. Department of Genetics, The Research Institute for Experimental Medicine, Istanbul University, 34093, Istanbul, Turkey

    Aris Cakiris & Duran Ustek

  3. Department of Surgery, Faculty of Veterinary Medicine, Istanbul University, 34320, Avcilar,, Istanbul, Turkey

    Ozlem Guzel & Kemal Altunatmaz

  4. Department of Gastroenterology, Gaziantep American Hospital, 27300, Gaziantep, Turkey

    Muzaffer Erturk

  5. Department of Physiology, Faculty of Veterinary Medicine, Istanbul University, 34320, Avcilar,, Istanbul, Turkey

    Ibrahim Akyazi

  6. Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, 34098, Cerrahpasa,, Istanbul, Turkey

    Hayriye Cicekci

  7. Department of Biochemistry, Faculty of Veterinary Medicine, Dicle University, Diyarbakır, Turkey

    Mehmet Hanifi Durak

  8. Food Technology Programme of Vocational High School, Faculty of Veterinary Medicine, Istanbul University, 34320, Avcilar,, Istanbul, Turkey

    Sema Sandikci-Altunatmaz

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  1. Ahmet Gulcubuk
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Correspondence to Ahmet Gulcubuk.

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Gulcubuk, A., Haktanir, D., Cakiris, A. et al. The effects of resveratrol on tissue injury, oxidative damage, and pro-inflammatory cytokines in an experimental model of acute pancreatitis. J Physiol Biochem 70, 397–406 (2014). https://doi.org/10.1007/s13105-014-0317-4

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  • DOI: https://doi.org/10.1007/s13105-014-0317-4

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