Abstract
There is an exponential growing attention in developing new micro/nano-delivery systems for controlled delivery of bioactive agents, to control cellular functions modulation in a more effective manner. In this study, the effect of dexamethasone encapsulated in calcium carbonate microcubes on proliferation and osteogenic activity of human osteoblasts in vitro was investigated. Dexamethasone-loaded calcium carbonate microcubes are suggested for supportive controlled release and improving osteogenic activity of low dose of dexamethasone. The calcium carbonate microcubes have a cubic structure of about 2 µm and represent a good performance in molecular delivery systems for bone tissue engineering thanks to their potential to mimic the composition, structure, and properties of native bone. The presence of polyelectrolyte multilayers that covered the calcium carbonate microcubes allowed to modulate the release kinetics of active dexamethasone over time. Our dexamethasone-loaded calcium carbonate microcubes provide a simple and easy way to allow controlled release of dexamethasone or other active agents in scaffolds for bone tissue engineering applications.
Similar content being viewed by others
References
Addadi L, Raz S, Weiner S (2003) Taking advantage of disorder: amorphous calcium carbonate and its role in biomineralization. Adv Mater 15:959–970
Anitua E, Sanchez M, Orive G, Andia I (2008) Delivering growth factors for therapeutics. Trends Pharmacol Sci 29:37–41
Dietrich JW, Canalis EM, Maina DM, Raisz LG (1979) Effects of glucocorticoids on fetal rat bone collagen synthesis in vitro. Endocrinology 104:715–721
Doherty MJ, Schlag G, Schwartz N, Mollan RAB, Nolan PC, Wilson DJ (1994) Biocompatability of xenogeneic bone, commercially available coral, a bioceramic and tissue sealant for human osteoblasts. Biomaterials 15:601–608
Guillemin G, Fournie J, Patat JL, Chetail M (1981) Fate of a fragment of madrepore coral skeleton implanted in the diaphysis of long bones in dogs CR Sconces. Acad Sci III 293:371–376
Longobardi L, O’Rear L, Aakula S, Johnstone B, Shimer K, Chytil A, Horton WA, Moses HL, Spagnoli A (2006) Effect of IGF-I in the chondrogenesis of bone marrow mesenchymal stem cells in the presence or absence of TGF-beta signaling. J Bone Miner Res 21:626–636
Park JS, Yang HN, Woo DG, Chung HM, Park KH (2009) In vitro and in vivo chondrogenesis of rabbit bone marrow-derived stromal cells in fibrin matrix mixed with growth factor loaded in nanoparticles. Tissue Eng 15:2163–2175
Santo VE, Gomes ME, Mano JF, Reis RL (2012a) From nano-to macro-scale: nanotechnology approaches for spatially controlled delivery of bioactive factors for bone and cartilage engineering. Nanomedicine 7:1045–1066
Santo VE, Gomes ME, Mano JF, Reis RL, Tabata (2012b) Chitosan–chondroitin sulphate nanoparticles for controlled delivery of platelet lysates in bone regenerative medicine. J Tissue Eng Regen Med 6:330
Zhang S, Uludag H (2009) Nanoparticulate systems for growth factor delivery. Pharm Res 26:561–1580
Acknowledgments
This study was supported by MAAT-Nanotecnologie molecolari per la salute dell’uomo e l’ambiente and Form@Beyond-Nano projects. No writing assistance was utilized in the production of this manuscript. The authors have no competing interests to disclose.
Author information
Authors and Affiliations
Corresponding author
Additional information
I. E. Palamà and V. Arcadio contributed equally.
Rights and permissions
About this article
Cite this article
Palamà, I.E., Arcadio, V., Cortese, B. et al. Dexamethasone delivery with coated calcium carbonate microcubes for sustained growth of osteoblasts. Rend. Fis. Acc. Lincei 26 (Suppl 2), 239–244 (2015). https://doi.org/10.1007/s12210-015-0430-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12210-015-0430-5